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Chromatin Immunoprecipitation

A significant epigenetic process that controls gene expression is chromatin modifications. Studying chromatin (or fragments of the DNA-protein complex) allows you to specify the types of proteins present within a genomic region, understand how these proteins interact with DNA, and identify the nucleic acid sequence and structure needed to assemble these complexes. DNA-protein interactions within a cell are crucial for correct gene regulation. They can determine whether proteins such as modified histones or transcription factors bind to a certain region of the DNA in a living cell.

General Chromatin Immunoprecipitation

Chromatin immunoprecipitation (ChIP) is an antibody-based method used for determining the location of DNA binding sites on the genome for a particular protein of interest. This technique is a convenient means for studying protein-DNA interactions that occur inside the nucleus of cells and for understanding cellular processes. Downstream applications of ChIP include ChIP-sequencing , ChIP-PCR, and ChIP-on-chip (microarrays).

EpiQuik Chromatin Immunoprecipitation Kit
EpiQuik Chromatin Immunoprecipitation Kit
EpiQuik Chromatin Immunoprecipitation Kit
EpiQuik Chromatin Immunoprecipitation Kit for Tissue
EpiQuik Chromatin Immunoprecipitation Kit for Tissue
EpiQuik Chromatin Immunoprecipitation Kit for Tissue
EpiQuik Chromatin Immunoprecipitation Kit for Plants
EpiQuik Chromatin Immunoprecipitation Kit for Plants
ChromaFlash One-Step ChIP Kit
ChromaFlash One-Step ChIP Kit
ChromaFlash One-Step Magnetic ChIP Kit
ChromaFlash One-Step Magnetic ChIP Kit
ChromaFlash High Sensitivity ChIP Kit
ChromaFlash High Sensitivity ChIP Kit

in vitro Protein-DNA Interaction

Measurement of direct interactions between protein and DNA in vitro has an advantage in analyzing the binding of different transcription factors to specific DNA consensus sequences located in the gene promoters. By investigating protein-DNA interaction in vitro, it is possible to identify the genetic targets of DNA, which leads to a better understanding of cellular processes.

Methyl-Histone Chromatin IP

Chromatin immunoprecipitation is a powerful technique for studying protein-DNA interaction in vivo, and can be coupled with various downstream applications to profile and map histone methylation patterns.

Acetyl-Histone Chromatin IP

Chromatin immunoprecipitation can be used as a tool to identify activated genes associated with acetylated histones, and can be coupled with qualitative and quantitative PCR, MSP, DNA sequencing, and Southern blot as well as DNA microarrays to further profile or map histone acetylation patterns.

Methyl-DNA Binding Protein ChIP

Methyl-CpG-binding domain protein 2 (MBD2) is a member of the MBD protein family and has been shown to catalyze demethylation by direclty removing methyl groups from 5-methylcytosine residues in DNA. Chromatin immunoprecipitation would be useful in the identification of silenced genes associated with MBD2, and can be combined with qualitative and quantitative PCR, MSP, DNA sequencing, DNA microarrays, and Southern blot to profile or map MBD2 binding patterns.

Chromatin Preparation

Chromatin is a combination of DNA and proteins that packages DNA into a smaller volume to fit in the cell, helps to prevent DNA damage, and controls gene expression and DNA replication. Proper isolation of chromatin samples is an important factor in assays for studying protein-DNA interactions.

Chromatin Accessibility

The accessibility of regulatory elements in chromatin is critical for many aspects of gene regulation. Nucleosomes positioned over regulatory elements inhibit access of transcription factors to DNA. To elucidate the role of the interactions between chromatin and transcription factors, it is crucial to determine chromatin accessibility through mapping of the nucleosome positioning along the genome.

MethylFlash Kits

BioCat Epigenetics Research

Use Epigentek's MethylFlash Kits for the quantification of epigenetic parameters.

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