microRNA Profiling by qPCR
microRNA qPCR Arrays
High-throughput microRNA Expression Profiling
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression at the post-transcriptional level. Usually 21-23 nucleotides in length, miRNAs are important modulators in cellular pathways and are highly conserved in eukaryotic organisms. Irregularities in miRNA-regulated gene expression have been found to be associated with cancers, cardiovascular disorders and a variety of other diseases.
miProfile miRNA qPCR Arrays
The miProfile miRNA PCR Arrays are designed for profiling the expression of pre-defined sets of miRNAs, such as whole genome, cancer-related or disease-related miRNAs, in various tissues or cells. The cancer-related or disease-related miRNAs are carefully chosen for their close correlation with a specific cancer or disease based on a thorough literature search of peer-reviewed publications. The resulting differential expression of profiled miRNAs help researchers to identify those miRNAs that are of biological significance and importance relevant to their research. Each 96-well plate contains up to 84 pairs of PCR primers (forward: miRNA-specific primer; reverse: universal primer) and 12 wells of different types of controls, which are used to monitor the efficiency of the entire experimental process – from reverse transcription to qPCR reaction.
Each miRNA-specific primer used in the qPCR arrays has been experimentally validated to yield a single dissociation curve peak and to generate a single amplification of the correct size for the targeted miRNA. A cDNA pool, containing reverse transcript products of 10 different tissue total RNAs, was used as the qPCR validation template.
Universal real-time PCR conditions were developed for easy profiling and analysis of miRNA expression in a high-throughput fashion.The All-in-One™ miRNA First-Strand cDNA Synthesis Kits and qPCR Mix Kits are the recommended and supported RT-PCR reagents for use with the miProfile miRNA qPCR arrays. These reagents have been optimized to produce high sensitivity, efficiency, and specificity.
- Profile whole genome, cancer-related or disease-related microRNAs
- Validated primers designed with proprietary algorithm
- Detect microRNA from as little as 10 pg of small RNA or 20 pg of total RNA
- Distinguish miRNAs with single nucleotide mismatches. Each primer set has been experimental validated for specific amplification
- Simultaneously detect microRNAs at different expression levels
- High reproducibility (R2 > 0.99) for inter-array and intra-array replicates
Five qPCR array formats (A, B, C, D, and E) suitable for use with the following real-time PCR instruments are provided. Before placing an order, please take care to choose the right kit version which is compatible with your instrument.
How It Works
microRNA qPCR Array Experimental Workflow
microRNA RT-PCR Mechanism
Linear Range and Sensitivity (Total RNA)
Single Nucleotide Mismatches