The CRISPR/Cas9 system can be used for knocking out gene expression in vivo or in vitro by using the combination of a sgRNA (single guide RNA) along with Cas9 (dCas9) nuclease. Achieve permanent 100% knockout in your cell line by using Cellecta´s lentivirus-based CRISPR system. Expression of the sgRNA and Cas9 are stable and can be used in dividing or non-dividing cells or whole model organisms.
A comprehensive collection of linearized, ready-to-use sgRNA cloning vectors developed by Cellecta and OriGene as well as different control vectors (non-targeting, sgCopGFP, sgPCNA, sgPOLR2L) is offered.
The Two Vector CRISPR/Cas9 System is used to perform functional knockout screens in vivo or in vitro by expressing sgRNA (single guide RNA) and Cas9 nuclease from different lentiviral vectors. It allows for faster knockout of the target gene in cells by first selecting cells expressing high levels of Cas9. This system is recommended when using complex sgRNA libraries in screening approaches.
The Single Vector CRISPR/Cas9 System comprises all-in-one constructs expressing sgRNA and Cas9 nuclease from the same vector.