seqWell Kits Comparison Guide

Tagmentation-based NGS DNA Library Preparation

Product	ExpressPlex™ 2.0	purePlex™	plexWell™	MosaiX™	LongPlex™
Primary Applications	Plasmid and amplicon sequencing Microbial/small genome WGS	Low frequency variants in plasmids and amplicons Microbial/small genome WGS Low coverage human/large genome WGS Metagenomic sequencing	Low frequency variants in plasmids and amplicons Microbial/small genome WGS Low coverage human/large genome WGS	Human whole genome sequencing (WGS) Human whole exome sequencing (WES) Human germ line target capture panels Plant & animal WGS and target capture panels	Long read sequencing Microbial/small genome WGS Targeted hybrid capture Metagenomic sequencing Low pass, large genome
Transposase Enzyme	TnX	Hyperactive Tn5	Hyperactive Tn5	TnX	Hyperactive Tn5
Sample Types	Amplicons (>350 bp), Plasmids Colony PCR, RCA, Genomic DNA	Amplicons (>500 bp), Plasmids, Genomic DNA	Amplicons (>500 bp), Plasmids, Genomic DNA	Genomic DNA	Genomic DNA
Total Prep Time (hands-on time)	100 min (30 min)	155 min (45 min)	190 min (55 min)	90 min (35 min)	105-225 min* (40 min)
Input Mass	1 - 40 ng	5 - 50 ng (based on batch size)	3 - 30 ng, 5 - 25 ng* 10 ng average	50 - 100 ng	150 - 500 ng*
Indexing Method	CDI	UDI	CDI	Any tagmentation-compatible indexing or custom primers	UDI
Batch Range	8-96 or 384*	8-24	96	1-96	1-24*
Total Samples	96 / 384 / custom	96	96 / 384	24 / 96	96
Number of Available Unique Index Combinations	Up to 6144	384	96 / 2304	N/A	96
Paired-End Reads (Clusters) per Sample Supported	≤4 million	≤20 million	≤4 million, ≤20 million*	≥400 million	NA
Sequencing Platform	Illumina	Illumina	Illumina	Illumina	PacBio

*Depending on the protocol