Bacterial EV Isolation Kit

Product Description

The Only Column-Based Kit for Both Gram-Positive and Gram-Negative Bacterial EVs

Isolate intact bacterial extracellular vesicles (30-400 nm) from culture media in 20 minutes—no ultracentrifugation, no overnight protocols, no mechanical stress that destroys vesicle cargo.

Why researchers choose this kit:

• Both bacterial classes: Works across Gram-positive and Gram-negative bacteria; no need for separate isolation methods
• Intact vesicles, preserved cargo: Slurry-based capture maintains RNA, protein, and lipid integrity for functional studies
• Flexible input volumes: Process 5-35 mL culture media - scale to your sample requirements
• Publication-ready purity: Compatible with NGS, qPCR, proteomics, functional assays, and downstream RNA isolation

Applications:

• Microbiome signaling and gut-brain axis research
• Host-pathogen interaction studies
• OMV-based vaccine development
• Bacterial communication mechanisms

Complete workflow integration:

Pair this kit with Norgen's Exosomal RNA Isolation Kit for cargo profiling. From isolation to characterization to analysis; BioCat provides the complete solution.

What you avoid:

• Hours of ultracentrifugation setup and processing
• Batch-to-batch variability from mechanical shearing
• Phenol/chloroform extractions and protease treatments
• Overnight precipitation protocols

Technical Specs:

• Size range: 30-400 nm extracellular vesicles
• Input volume: 5-35 mL bacterial culture media
• Processing time: ~20 minutes
• Method: Proprietary slurry-based capture (no special equipment required)

Supporting Data

Figure 1: Average particle concentration by size for EV isolation done from 10 mL of bacterial culture media at 5× dilution, with error bars. This graph shows the average concentration of particles (particles/mL) versus size (nm), measured using nanoparticle tracking analysis (NTA). Error bars indicate ±1 standard error of the mean (SEM), reflecting variability across replicates. The main distribution peaks near 30-150 nm support the presence of nanoscale particles consistent with EVs in the sample.

Figure 2: Scattered light intensity versus particle size for EV isolation done from 10 mL of bacterial culture media at 5× dilution. This graph presents the mean light scattering intensity (arbitrary units) as a function of particle size, measured using nanoparticle tracking analysis (NTA). The intensity increases with particle size. It shows relative particle size contributions and confirms the presence of 30-150nm particles (EVs).

Figure 3: Average microRNA concentration obtained from EV RNA isolation across varying bacterial media sample volumes. This bar graph shows the average microRNA concentration (ng/μL), measured using a Qubit fluorometric assay, following EV RNA isolation from bacterial media samples of increasing starting volumes (5 mL, 10 mL, 20 mL and 35 mL). Each bar represents the mean concentration for a given volume, with error bars indicating standard deviation across replicates. A positive trend is observed, with higher initial sample volumes yielding higher microRNA concentrations. This suggests that increasing the volume of processed media enhances the total recovery of microRNA from EV isolations.

Figure 4: Average quantification cycle (Cq) values from 16S rRNA qPCR across varying bacterial media sample volumes used for EV RNA isolation. This bar graph shows the average Cq values from quantitative PCR targeting the bacterial 16S rRNA gene following EV RNA extraction from different starting volumes of bacterial media (5 mL, 10 mL, 20 mL and 35 mL). Error bars represent standard deviation across replicates. A lower Cq value indicates higher template abundance. As sample volume increases, the Cq values decrease, suggesting a greater yield of bacterial RNA as more input volume is processed. This trend supports the use of larger starting volumes to improve EV RNA recovery.