Custom Lentiviral shRNA Expression Construct, plasmid only

Custom shRNA Knockdown Constructs

Product Description

To make an lentiviral shRNA construct, we only need to know the RefSeq number. Cellecta typically designes and clones 3-5 constructs that target your transcript. The proprietary shRNA design delivers approximately 70% shRNAs that generate >70% transcript knockdown in standard cell lines as measured by qRT-PCR so it is likely that at least 2 of 3 or 3 of 5 constructs will be effective.

Cellecta makes the inserts, clones them, and sequences the constructs to verify correct construction. You receive several of the best-designed constructs to test and characterize to see which best matches your experimental needs. We can provide the plasmid constructs, or you have the option to also package the constructs as ready-to-transduce lentiviral particles.

This level cannot be guaranteed for any specific target, and the percentage of highly effective shRNAs will vary from target to target.

Choose the Vector

You can order the shRNA cloning in the standard DECIPHER vector or in a modified version. Browse Vector Options for vector information for standard cloning vectors, including shRNA cassettes and primer maps, sequences, GenBank files, and vector maps.

Fig.1. pRSI lentiviral shRNA expression vector.

Performance Data

Fig.2. Percentage knockdown for 120 shRNA constructs targeting 8 genes.

Related Service

Lentiviral sg/shRNA Packaging Service