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SGI-DNA

SGI-DNA

SGI-DNA, a wholly owned subsidiary of Synthetic Genomics, Inc (SGI), was founded in 2013 and is headquartered in La Jolla, CA. Building on the scientific advancements and breakthroughs from leading scientists such as J. Craig Venter, Ham Smith, Clyde Hutchison, Dan Gibson and their teams, SGI-DNA utilizes unique and proprietary DNA technologies to produce complex synthetic genes and reagents.

Gibson Assembly® Cloning NEW!

The Gibson Assembly® method is a cloning procedure that allows the cloning of two or more fragments without the need for restriction enzyme digestion or compatible restriction sites. Instead, user-defined overlapping ends are incorporated into the fragments to allow the seamless joining of adjacent fragments. This innovative approach to creating both simple and complex constructs was first published in 2008 by Daniel Gibson and colleagues*. Since that time, the Gibson Assembly® method has been cited in over 3000 peer-reviewed publications. Due to its many advantages over traditional restriction enzyme cloning, Gibson Assembly® cloning is rapidly becoming the preferred method for cloning DNA into plasmids and bacterial artificial chromosomes (BAC) in many laboratories.

*Gibson et al. Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science. 2008 Feb 29;319(5867):1215-20.

Vmax™ Express Competent Cells

Recombinant Protein Expression: Fast Growth and High Yields of Soluble Protein

  • Fastest growth rate of any known bacterial host with a doubling time of ~14 minutes
  • Produces up to twice the biomass of E. coli with up to 4x the protein
  • Enables expression of high levels of soluble recombinant proteins
  • Tightly regulated, IPTG-inducible T7 promoter system
  • Compatible with commonly used E. coli expression vectors, antibiotics, and growth media
  • Protein expression workflow similar to that of E. coli
  • Tolerates induction over wide range OD’s (0.3 to 1.0) and IPTG concentrations (0.5 to 1.0 mM)
  • Efficient protein expression up to 24-hours post-induction
  • Rapid results - Go from cells to large-scale culture ready for protein purification in three days

Vmax™ Express is a novel, fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. This rationally engineered, next-generation prokaryotic protein expression system can serve as a replacement for slow growing E. coli systems that are prone to low yields and the expression of proteins as insoluble inclusion bodies. Vmax™ cells are derived from the marine microorganism, Vibrio natriegens. This gram-negative, non-pathogenic bacterium has a doubling time twice that of E. coli and robust transcription and translation systems that support this rapid growth rate. This means that Vmax™ Express can produce greater amounts of biomass and generate large amounts of protein in a shorter time period.

Applications

  • Lab scale expression of recombinant proteins
  • Large scale expression of recombinant proteins
  • Expression of proteins that express at low levels or as inclusion bodies in E. coli
  • Expression of active proteins
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