CRISPR Cas9 Lentiviral Guide RNA Cloning Vectors and Control Constructs

The CRISPR-Cas9 system can be used for knocking out gene expression in vivo or in vitro by using a combination of an sgRNA (single guide RNA) along with Cas9 (dCas9) nuclease. Achieve permanent 100% knockout in your cell line by using Cellecta´s lentiviral-based CRISPR system. Expression of the sgRNA and Cas9 are stable and can be used in dividing or non-dividing cells or whole model organisms.

Example Data: Knock Out of copGFP Gene in HEK293 Cells


Several sgRNAs targeting the copGFP gene were designed and individually transduced into HEK293 cells stably expressing copGFP. After 9 days, cells were imaged for GFP fluorescence. For each sgRNA tested, GFP expression was abolished in at least 70% of transduced cells.


Cellecta are offering a Two-Vector as well as a One-Vector CRISPR-Cas9 System.


Two Vector CRISPR-Cas9 System

The Two Vector CRISPR-Cas9 system is used to perform functional knockout screens in vivo or in vitro by expressing sgRNA (single guide RNA) and Cas9 nuclease from different lentiviral vectors.

This system is recommended when using complex sgRNA libraries in screening approaches.

• Pre-transduce with Cas9 lentivirus to decrease noise in your knockout screen
• Make high-titer sgRNA lentivirus for efficient transduction
• Vectors contain different antibiotic resistance genes to ensure stability and easy selection
• Tet-inducible and constitutive U6 expression available


The sgRNA vector is also available with GFP instead of RFP as well as with an inducible U6 promoter and the CMV promoter controlling the expression of TetRep, RFP or GFP, and Puro

Vector Names:

pRSG17-U6-(sg)-UbiC-TagGFP2-2A-Puro
pRSGT16-U6Tet-(sg)-CMV-TetRep-2A-tagRFP-2A-Puro
pRSGT17-U6Tet-(sg)-CMV-TetRep-2A-tagGFP2-2A-Puro
Please see link below for the whole range.

New: These vectors are available with HEATtracr scaffold - see product list below - you only have to clone the 24-mer sgRNA sequence, and will take advantage of Cellecta´s HEAT sgRNA design substantially improving specificity and efficiency: HEAT sgRNA Design!


More vectors, e.g. with EF1 promoter, are also available.
Please inquire, when you do not find the promoter or marker combination of your choice.


The Cas9 Expression Vector (see link below) is also available with blast instead of hygro resistance.


Single Vector CRISPR-Cas9 System: Stable Expression of Cas9 and sgRNA All-in-One-Vector
• Co-transduction not necessary since sgRNA and Cas9 nuclease are expressed in one vector
• Lentiviral vectors integrate into the host cell genome and are passed onto daughter cells
• Cells can be treated, grown for several passages, frozen and thawed - the lentiviral construct remains in the host cells
• Vector contains antibiotic selection to ensure stability





Two popular one-in-all sgRNA cloning vectors from Cellecta.
Please see link below for the whole range.

More vectors, e.g. with EF1 or SV40 promoter, are also available.
Please inquire, when you do not find the promoter or marker combination of your choice.



You can purchase all gRNA vectors linearized and ready-to-use for cloning, as well as various controls as plasmid DNAs or packaged lentiviral particles.

---

Related Links

Cas9 Expression Vector
Functional CAS9 Activity Kit
Overview: Cellecta´s sgRNA Cloning Vectors
Please inquire for vectors with other promoters/markers

 

 

Description	Cat#	Size	Price
pRSGCCP-U6-(sg)-CMV-Cas9-2A-Puro lentiviral sgRNA-Cas9 vector (One Vector System), linearized - ready for cloning (500 ng)	SVCRU6CCP-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hygro lentiviral sgRNA-Cas9 vector (One Vector System), linearized - ready for cloning (500 ng)	SVCRU6CCH-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGCCB-U6-(sg)-CMV-Cas9-2A-Blast lentiviral sgRNA-Cas9 vector (One Vector System), linearized - ready for cloning (500 ng)	SVCRU6CCB-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGCCN-U6-(sg)-CMV-Cas9-2A-Neo lentiviral sgRNA-Cas9 vector (One Vector System), linearized - ready for cloning (500 ng)	SVCRU6CCN-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGCCG-U6-(sg)-CMV-Cas9-2A-TagGFP2 lentiviral sgRNA-Cas9 vector (One Vector System), linearized - ready for cloning (500 ng)	SVCRU6CCG-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGCCR-U6-(sg)-CMV-Cas9-2A-TagRFP lentiviral sgRNA-Cas9 vector (One Vector System), linearized - ready for cloning (500 ng)	SVCRU6CCR-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSG17-U6-(sg)-UbiC-TagGFP2-2A-Puro lentiviral sgRNA vector (Two Vector System), linearized - ready for cloning (500 ng)	SVCRU617-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSG20-U6-(sg)-CMV-TagRFP-2A-Puro lentiviral sgRNA vector (Two Vector System), linearized - ready for cloning (500 ng)	SVCRU620-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSG21-U6-(sg)-CMV-TagGFP2-2A-Puro lentiviral sgRNA vector (Two Vector System), linearized - ready for cloning (500 ng)	SVCRU621-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGEP-U6-(sg)-EF1-Puro lentiviral sgRNA vector (Two Vector System), linearized - ready for cloning (500 ng)	SVCRU6EP-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGT16-U6Tet-(sg)-CMV-TetRep-2A-TagRFP-2A-Puro, lentiviral inducable sgRNA vector (Two Vector System), linearized - ready for cloning (500 ng)	SVCRU6T16-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGT17-U6Tet-(sg)-CMV-TetRep-2A-TagGFP2-2A-Puro, lentiviral inducable sgRNA vector (Two Vector System), linearized - ready for cloning (500 ng)	SVCRU6T17-L-CT	50 rxns	657 €	DETAILS	Add to Cart
pRSGTEP-U6Tet-(sg)-EF1-TetRep-2A-Puro, lentiviral inducable sgRNA vector (Two Vector System), linearized - ready for cloning (500 ng)	SVCRU6TEP-L-CT	50 rxns	657 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSG16-(sg)-UbiC-RFP-Puro (Two Vector System), Plasmid DNA	SGCTL-COP-pRSG16-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSG16-(sg)-UbiC-RFP-Puro (Two Vector System), packaged lentiviral particles	SGCTL-COP-pRSG16-V-GVO-CT	1 x 10e7 TU	657 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSG17-(sg)-UbiC-GFP-Puro (Two Vector System), Plasmid DNA	SGCTL-COP-pRSG17-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSG17-(sg)-UbiC-GFP-Puro (Two Vector System), packaged lentiviral particles	SGCTL-COP-pRSG17-V-GVO-CT	1 x 10e7 TU	657 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGT16-(sg)-CMV-TetR-RFP-Puro (Two Vector System), Plasmid DNA	SGCTL-COP-pRSGT16-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGT16-(sg)-CMV-TetR-RFP-Puro (Two Vector System), packaged lentiviral particles	SGCTL-COP-pRSGT16-V-GVO-CT	1 x 10e7 TU	657 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGT17-(sg)-CMV-TetR-GFP-Puro (Two Vector System), Plasmid DNA	SGCTL-COP-pRSGT17-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGT17-(sg)-CMV-TetR-GFP-Puro (Two Vector System), packaged lentiviral particles	SGCTL-COP-pRSGT17-V-GVO-CT	1 x 10e7 TU	657 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSG16-(sg)-UbiC-RFP-Puro (Two Vector System), Plasmid DNA	SGCTL-NT-pRSG16-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSG16-(sg)-UbiC-RFP-Puro (Two Vector System), packaged lentiviral particles	SGCTL-NT-pRSG16-V-GVO-CT	1 x 10e7 TU	657 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSG17-(sg)-UbiC-GFP-Puro (Two Vector System), Plasmid DNA	SGCTL-NT-pRSG17-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSG17-(sg)-UbiC-GFP-Puro (Two Vector System), packaged lentiviral particles	SGCTL-NT-pRSG17-V-GVO-CT	1 x 10e7 TU	657 €	DETAILS	Add to Cart
CRISPR Control: PCNA sgRNA (lethal) in lentiviral sgRNA vector (Two Vector System), Plasmid DNA	SGCTL-PCNA-PX-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: POLR2L sgRNA (lethal) in lentiviral sgRNA vector (Two Vector System), Plasmid DNA	SGCTL-POLR2L-PX-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hyg (One Vector System), Plasmid DNA	SGCCTL-COP-pRSGCCH-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hyg (One Vector System), packaged lentiviral particles	SGCCTL-COP-pRSGCCH-V-GVO-CT	1 x 10e6 TU	657 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Puro (One Vector System), Plasmid DNA	SGCCTL-COP-pRSGCCP-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: copGFP sgRNA (non-targeting) in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Puro (One Vector System), packaged lentiviral particles	SGCCTL-COP-pRSGCCP-V-GVO-CT	1 x 10e6 TU	657 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hyg (One Vector System), Plasmid DNA	SGCCTL-NT-pRSGCCH-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Hyg (One Vector System), packaged lentiviral particles	SGCCTL-NT-pRSGCCH-V-GVO-CT	1 x 10e6 TU	657 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSGCCG-U6-(sg)-CMV-Cas9-2A-GFP (One Vector System), Plasmid DNA	SGCCTL-NT-pRSGCCG-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Puro (One Vector System), Plasmid DNA	SGCCTL-NT-pRSGCCP-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSGCCG-U6-(sg)-CMV-Cas9-2A-GFP (One Vector System), packaged lentiviral particles	SGCCTL-NT-pRSGCCG-V-GVO-CT	1 x 10e6 TU	657 €	DETAILS	Add to Cart
CRISPR Control: Non Targeting Scrambled sgRNA in pRSGCCH-U6-(sg)-CMV-Cas9-2A-Puro (One Vector System), packaged lentiviral particles	SGCCTL-NT-pRSGCCP-V-GVO-CT	1 x 10e6 TU	657 €	DETAILS	Add to Cart
CRISPR Control: PCNA sgRNA (lethal) in lentiviral sgRNA-Cas9 vector (One Vector System), Plasmid DNA	SGCCTL-PCNA-PX-CT	25 ug	400 €	DETAILS	Add to Cart
CRISPR Control: POLR2L sgRNA (lethal) in lentiviral sgRNA-Cas9 vector (One Vector System), Plasmid DNA	SGCCTL-POLR2L-PX-CT	25 ug	400 €	DETAILS	Add to Cart