• Multiplex - A single assay allows the measurement of the activities of 96 TFs
• Quantitative comparison - The difference of two samples can be quantitatively analyzed and compared
• Simple procedures - Probe incubation, spin column separation, plate hybridization, and HRP detection
• No special requirement - Capital instrument such as Luminex is not needed
Signosis’ TF activation profiling plate array is used for monitoring the activation of multiple TFs simultaneously. In the technology, a series of biotin-labeled probes are made based on the consensus sequences of TF DNA-binding sites. When the probe mix incubate nuclear extracts, individual probes will find its corresponding TF and form TF/probe complexes, which can be easily separated from free probes through a simple spin column purification method. The bound probes are detached from the complex and analyzed through hybridization with a plate; each well is specifically pre-coated with complementary sequences of the probes.