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SARS-CoV-2 Variant Research

Like other viruses, SARS-CoV-2 changes over time. Most changes have little to no impact on the virus’ properties. Some changes, however, may have an effect on its transmissibility and spread, antigenicity or virulence. This in turn might affect the associated disease severity or the performance of vaccines.
BioCat offers a variety of tools to accelerate critical research and enhance understanding of the biology of SARS-CoV-2 variants.

SARS-CoV-2 Variant Recombinant Proteins

Choose from our comprehensive collection of SARS-CoV-2 variant recombinant proteins, including recombinant spike protein and nucleocapsid protein mutants from the Alpha, Beta, Gamma and Delta variants, and evaluate the efficacy of antibodies and vaccination.

SARS-CoV-2 Variant Spike Protein Pseudotyped Lentivirus

As potentially more transmissible variants of SARS-CoV-2 emerge, understanding how mutations in the spike protein impact SARS-CoV-2 behavior is critical. SARS-CoV-2 spike protein-pseudotyped lentivirus products generate a specially-packaged variant of standard lentivirus, whereby the commonly used vesicular stomatitis virus envelope glycoprotein G (VSV-G) is replaced by the spike (S) protein, truncated spike protein or spike protein mutants from SARS-CoV-2 variants like Alpha, Beta, Gamma and Delta. As a result, a range of SARS-CoV-2 studies can be conducted under biosafety level 2 conditions, including neutralization assays, studies of virus interactions with host surface proteins, and the development of vaccines and therapeutics.

SARS-CoV-2 Spike Protein Pseudotyped Lentivirus Packaging Mixes for packaging your own third-generation lentivectors as well as Premade SARS-CoV-2 Spike Protein Pseudotyped Lentiviral Particles are offered.

SARS-CoV-2 Spike Protein Pseudotyped Lentivirus Packaging Mixes

Designed to efficiently package third-generation lentivectors, the SARS-CoV-2 Spike Protein Pseudotyped Lentivirus Packaging Mixes speed and simplify the preparation of lentiviral particles pseudotyped with the SARS-CoV-2 spike glycoprotein. Replacing the standard VSV-G envelope protein with full-length spike, truncated spike or spike protein mutants from SARS-CoV-2 variants like Alpha, Beta, Gamma and Delta, these packaging mixes consist of three plasmids that produce all of the structural and replication proteins needed to transcribe and package a RNA copy of an expression lentivector into recombinant, “Spike” pseudotyped lentiviral particles. These particles can be used in vitro to infect cultured cells expressing human host factors like ACE2, the receptor for SARS-CoV-2 in multiple tissue types, or in vivo in animal models.

Lentivirus pseudotyping as a custom service is also provided, please contact us for more information.


pPACK-SPIKE SARS-CoV-2 S Pseudotype Lentivector Packaging Mix CVD19-500A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE SARS-CoV-2 S Pseudotype Lentivector Packaging Mix (XL) CVD19-510A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE Combo Kit, includes Cat# CVD19-500A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-520A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE D614G, SARS-CoV-2 S Pseudotype - D614G Mutant - Lentivector Packaging Mix CVD19-530A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE D614G, SARS-CoV-2 S Pseudotype - D614G Mutant - Lentivector Packaging Mix (XL) CVD19-540A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE D614G Combo Kit, includes Cat# CVD19-530A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-550A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE N501Y SARS-CoV-2 S Pseudotype - N501Y Mutant - Lentivector Packaging Mix CVD19-560A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE N501Y SARS-CoV-2 S Pseudotype - N501Y Mutant - Lentivector Packaging Mix (XL) CVD19-570A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE N501Y Combo Kit, includes Cat# CVD19-560A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-575A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE B.1.351 RBD Mutations, SARS-CoV-2 S Pseudotype - B.1.351 (S. African) Variant - RBD Mutations - Lentivector Packaging Mix CVD19-580A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE B.1.351 RBD Mutations, SARS-CoV-2 ''S'' Pseudotype - B.1.351 (S. African) Variant - RBD Mutations - Lentivector Packaging Mix (XL) CVD19-585A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE B.1.351 RBD Mutations Combo Kit, includes Cat# CVD19-580A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-589A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE Alpha (B.1.1.7), SARS-CoV-2 S Pseudotype - Alpha (B.1.1.7) Variant - Lentivector Packaging Mix CVD19-590A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE Alpha (B.1.1.7), SARS-CoV-2 S Pseudotype - Alpha (B.1.1.7) Variant - Lentivector Packaging Mix (XL) CVD19-595A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE Alpha Combo Kit, includes Cat# CVD19-590A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution CVD19-599A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE B.1.351 S1 Mutations, SARS-CoV-2 S Pseudotype - B.1.351 (S. African) Variant - S1 Mutations (RBD+D614G) - Lentivector Packaging Mix CVD19-600A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE B.1.351 S1 Mutations, SARS-CoV-2 S Pseudotype - B.1.351 (S. African) Variant - S1 Mutations (RBD+D614G) - Lentivector Packaging Mix (XL) CVD19-605A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE B.1.351 S1 Mutations Combo Kit, includes Cat# CVD19-600A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-609A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE B.1.429, SARS-CoV-2 S Pseudotype - B.1.429 (CAL.20C) Variant - Lentivector Packaging Mix CVD19-610A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE B.1.429, SARS-CoV-2 S Pseudotype - B.1.429 (CAL.20C) Variant - Lentivector Packaging Mix (XL) CVD19-615A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE B.1.429 Combo Kit, includes Cat# CVD19-610A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-619A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE B.1.525, SARS-CoV-2 S Pseudotype - B.1.525 Variant - Lentivector Packaging Mix CVD19-620A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE B.1.525, SARS-CoV-2 S Pseudotype - B.1.525 Variant - Lentivector Packaging Mix (XL) CVD19-625A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE B.1.525 Combo Kit, includes Cat# CVD19-620A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-629A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE Gamma (P.1), SARS-CoV-2 S Pseudotype - Gamma (P.1) Variant - Lentivector Packaging Mix CVD19-630A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE Gamma (P.1), SARS-CoV-2 S Pseudotype - Gamma (P.1) Variant - Lentivector Packaging Mix (XL) CVD19-635A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE Gamma Combo Kit, includes Cat# CVD19-630A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution CVD19-639A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE Beta (B.1.351), SARS-CoV-2 S Pseudotype - Beta (B.1.351) Variant - Lentivector Packaging Mix CVD19-640A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE Beta (B.1.351), SARS-CoV-2 S Pseudotype - Beta (B.1.351) Variant - Lentivector Packaging Mix (XL) CVD19-645A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE Beta Combo Kit, includes Cat# CVD19-640A-1, plus PureFection Transfection Reagent (Cat# LV750A-1) and PEG-it Virus Concentration solution CVD19-649A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-SPIKE Delta (B.1.617.2), SARS-CoV-2 S Pseudotype - Delta (B.1.617.2) Variant - Lentivector Packaging Mix CVD19-650A-1-SBI 10 rxn(s)   786.00 €
pPACK-SPIKE Delta (B.1.617.2), SARS-CoV-2 S Pseudotype - Delta (B.1.617.2) Variant - Lentivector Packaging Mix (XL) CVD19-655A-1-SBI 25 rxn(s)   1547.00 €
pPACK-SPIKE Delta Combo Kit, includes Cat# CVD19-650A-1, plus PureFection Transfection Reagent and PEG-it Virus Concentration solution CVD19-659A-KIT-SBI 1 kit(s)   1312.00 €
pPACK-BALD Envelope-Free Lentivector Packaging Mix LV550A-1-SBI 10 rxn(s)   620.00 €
pPACK-BALD Envelope-Free Lentivector Packaging Mix (XL) LV555A-1-SBI 25 rxn(s)   1341.00 €
Lenti-Pac SARS-CoV-2 Full Length Spike protein-pseudotyped Lentivirus Packaging Kit (20 reactions) LT011-GC 20 rxn(s)   861.00 €
Lenti-Pac™ SARS-CoV-2 Full Length Spike protein-pseudotyped (D614G)Lentivirus Packaging Kit (20 reactions) LT015-GC 20 rxn(s)   861.00 €
Lenti-Pac SARS-CoV-2 Truncated Spike protein-pseudotyped Lentivirus Packaging Kit (20 reactions) LT021-GC 20 rxn(s)   861.00 €
Lenti-Pac™ SARS-CoV-2 Truncated Spike protein-pseudotyped (D614G)Lentivirus Packaging Kit, ER retention signal removed (20 reactions) LT017-GC 20 rxn(s)   861.00 €
Lenti-Pac SARS-CoV-2 Full Length Spike protein-pseudotyped (UK B.1.1.7 & D614G) Lentivirus Packaging Kit LT041-GC 20 rxn(s)   861.00 €
Lenti-Pac SARS-CoV-2 Full Length Spike protein-pseudotyped (South African B.1.351 & D614G) Lentivirus Packaging Kit LT044-GC 20 rxn(s)   861.00 €
Lenti-Pac SARS-CoV-2 Full Length Spike protein-pseudotyped (Brazil B.1.1.28.1) Lentivirus Packaging Kit LT047-GC 20 rxn(s)   861.00 €
SARS-CoV2-S Pseudotyped Lentiviral Packaging Mix (enough for ~12 10-cm2 plates to make ~1-2 x 10e7 TU) RSCOV2-SPACK-CT 250 ug   748.00 €
SARS-CoV2-S-D614G Pseudotyped Lentiviral Packaging Mix (enough for ~12 10-cm2 plates to make ~1-2 x 10e7 TU) RSCOV2-SDGPACK-CT 250 ug   748.00 €

How It Works

The SARS-CoV-2 Spike Protein Pseudotyped Lentivirus Packaging Kits are easy to use - simply co-transfect the packaging plasmids and your lentivector construct into lentiviral production cells, isolate pseudoviral particles with an optional concentration step, and conduct your studies.



Premade SARS-CoV-2 Spike Protein Pseudotyped Lentiviral Particles


HLUC-Lv201 Firefly Luciferase Lentifect SARS-CoV-2 full-length Spike-Pseudotyped Lentiviral Particles SP101-100-GVO-GC 100 ul (> 1x 10^5 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP001-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase Lentifect SARS-CoV-2 full-length D614G Spike-Pseudotyped Lentiviral Particles SP103-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 D614G Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP003-100-GVO-GC 100 ul (> 5x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP LentifectSARS-CoV-2 full-length N439K Spike-Pseudotyped Lentiviral Particles SP104-100-GVO-GC 100 ul (> 1x 10^5 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 N439K Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP004-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 full-length (D614G & N439K) Spike-Pseudotyped Lentiviral Particles SP105-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 (D614G & N439K) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP005-100-GVO-GC 100 ul (> 5x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 full-length (UK B.1.1.7 & D614G) Spike-Pseudotyped Lentiviral Particles SP106-100-GVO-GC 100 ul (> 1x 10^5 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 (UK B.1.1.7 & D614G) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP006-100-GVO-GC 100 ul (> 5x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 full-length (South African B.1.351 & D614G) Spike-Pseudotyped Lentiviral Particles SP107-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 (South African B.1.351 & D614G) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP007-100-GVO-GC 100 ul (> 5x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 full-length (Brazil P.1 & D614G) Spike-Pseudotyped Lentiviral Particles SP108-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 (Brazil P.1 & D614G) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP008-100-GVO-GC 100 ul (> 5x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 full-length (India B.1.617.1) Spike-Pseudotyped Lentiviral Particles SP109-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 (India B.1.617) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP009-100-GVO-GC 100 ul (> 1x 10^5 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 full-length (India B.1.617.2) Spike-Pseudotyped Lentiviral Particles SP111-100-GVO-GC 100 ul (> 1x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase + eGFP Lentifect SARS-CoV-2 (India B.1.617.2) Spike-Pseudotyped Lentiviral Particles, ER retention signal removed SP011-100-GVO-GC 100 ul (> 5x 10^6 TU/ml)   1090.00 €
HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Lentiviral Particles without envelope protein SP401-025-GVO-GC 25 ul   402.00 €
HLUC-Lv201 Firefly Luciferase Lentifect™ SARS-CoV-2 Lentiviral Particles without envelope protein (25 µl x 4 vial) SP401-100-GVO-GC 100 ul   965.00 €
SARS-CoV2-S Pseudotyped GFP Lentivirus (titered in Cellecta HeLa-Ace2 Cells) RSCOV2-SG-2-GVO-CT 2 mls (10^4 TU/ml)   1064.00 €
SARS-CoV2-S Pseudotyped GFP Lentivirus (titered in Cellecta HeLa-Ace2 Cells) RSCOV2-SG-10-GVO-CT 10 mls (10^4 TU/ml)   2077.00 €
SARS-CoV2-S Pseudotyped GFP Lentivirus (titered in Cellecta HeLa-Ace2 Cells) RSCOV2-SG-50-GVO-CT 50 mls (10^4 TU/ml)   4103.00 €
SARS-CoV2-S-D614G Pseudotyped GFP Lentivirus (titered in Cellecta HeLa-Ace2 Cells) RSCOV2-SDGG-2-GVO-CT 2 mls (10^4 TU/ml)   1064.00 €
SARS-CoV2-S-D614G Pseudotyped GFP Lentivirus (titered in Cellecta HeLa-Ace2 Cells) RSCOV2-SDGG-10-GVO-CT 10 mls (10^4 TU/ml)   2077.00 €
SARS-CoV2-S-D614G Pseudotyped GFP Lentivirus (titered in Cellecta HeLa-Ace2 Cells) RSCOV2-SDGG-50-GVO-CT 50 mls (10^4 TU/ml)   4103.00 €
VSV-G Pseudotyped GFP Lentivirus (titered in HeLa Cells) RVSV-GG-2-GVO-CT 2 mls (10^4 TU/ml)   746.00 €
VSV-G Pseudotyped GFP Lentivirus (titered in HeLa Cells) RVSV-GG-10-GVO-CT 10 mls (10^4 TU/ml)   1440.00 €
VSV-G Pseudotyped GFP Lentivirus (titered in HeLa Cells) RVSV-GG-50-GVO-CT 50 mls (10^4 TU/ml)   2135.00 €
SARS-CoV2-S Pseudotyped Luc Lentivirus (assayed using Cellecta HeLa-Ace2 Cells) RSCOV2-SL-2-GVO-CT 2 mls (~40 rxns)   1064.00 €
SARS-CoV2-S Pseudotyped Luc Lentivirus (assayed using Cellecta HeLa-Ace2 Cells) RSCOV2-SL-10-GVO-CT 10 mls (~200 rxns)   2077.00 €
SARS-CoV2-S Pseudotyped Luc Lentivirus (assayed using Cellecta HeLa-Ace2 Cells) RSCOV2-SL-50-GVO-CT 50 mls (~1000 rxns)   4103.00 €
SARS-CoV2-S-D614G Pseudotyped Luc Lentivirus (assayed using Cellecta HeLa-Ace2 Cells) RSCOV2-SDGL-2-GVO-CT 2 mls (~40 rxns)   1064.00 €
SARS-CoV2-S-D614G Pseudotyped Luc Lentivirus (assayed using Cellecta HeLa-Ace2 Cells) RSCOV2-SDGL-10-GVO-CT 10 mls (~200 rxns)   2077.00 €
SARS-CoV2-S-D614G Pseudotyped Luc Lentivirus (assayed using Cellecta HeLa-Ace2 Cells) RSCOV2-SDGL-50-GVO-CT 50 mls (~1000 rxns)   4103.00 €
VSV-G Pseudotyped Luc Lentivirus (assayed using HeLa Cells) RVSV-GL-2-GVO-CT 2 mls (~40 rxns)   746.00 €
VSV-G Pseudotyped Luc Lentivirus (assayed using HeLa Cells) RVSV-GL-10-GVO-CT 10 mls (~200 rxns)   1440.00 €
VSV-G Pseudotyped Luc Lentivirus (assayed using HeLa Cells) RVSV-GL-50-GVO-CT 50 mls (~1000 rxns)   2135.00 €

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