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Promoter Reporter Clones


10% OFF* GLuc-ON™ Promoter Reporter Clones

Using a secreted and robust Gaussia Luciferase (GLuc) as the reporter, GeneCopoeia GLuc-ON™ promoter clones are designed for promoter analysis by detecting the real-time activities of over 20,000 human and 18,000 mouse promoters using live cell assays.

Each transfection-ready promoter clone contains a 1.2-1.5 kb insert, corresponding to the 5'-flanking promoter sequence located approximately 1.5 kb upstream and up to 200 bp downstream of the transcription start site (TSS) of a specific gene. This insert is placed upstream of the GLuc reporter gene. Since the putative cis-acting enhancer elements are expected to exist in the cloned promoter region, the promoter luciferase activity observed during the reporter assay closely resembles the actual promoter regulation of these genes within human cells.

In addition to the dual-reporter system containing GLuc as the promoter reporter and Secreted Alkaline Phosphatase (SEAP) as the internal control for signal normalization, single-reporter vectors with GLuc or a fluorescent protein as the promoter reporter are available, see table below. GLuc-ON promoter reporter clones can be ordered as pre-designed or custom-built in one of our robust vector systems.

Special Price* Assay Kits

Depending on the reporter vector, the Secrete-Pair Dual Luminescence Assay Kit (dual reporter GLuc and SEAP) or the Secrete-Pair Gaussia Luciferase Assay Kit (single reporter GLuc) are recommended for promoter analysis using GeneCopoeia's GLuc-ON™ Promoter Reporter Clones.

*Expires 30 June 2019


How GLuc-ON Promoter Clones Work



Gaussia Luciferase

GLuc-ON promoter clones use a modified GLuc (mGLuc) as the reporter gene, which generates a highly stable signal and overcomes the quick signal decay commonly observed with humanized wild type GLuc (wtGLuc).

Signal stability of mGluc and wtGlucSignal stability of mGluc and wtGluc

Figure 1. Signal stability of mGLuc (blue) and wtGLuc (red). Left: assay buffer with a stabilizer; Right: regular assay buffer (Secrete-Pair™ dual luminescence assay kit)


Dual-Reporter System

Dual-reporter vectors are available for the GLuc-ON promoter clones. The secondary reporter, secreted Alkaline Phosphatase (SEAP), serves as an internal control. The dual-reporter system enables transfection normalization for accurate cross-sample comparison.

 

Figure 2. Normalized promoter activities in H1B1B and HEK293T cells. Dual-reporter promoter clones or controls were transfected into two cell lines in duplicates. Samples were analyzed 24 hrs (HEK293T) and 48 hrs (H1B1B) after transfection. NEG (containing non-promoter sequence) and EMPTY (no promoter in the vector) are negative controls.


Vectors

All Promoter Reporter Clones are available in the vectors listed below. Find your gene specific construct using our clone search:

Vector Reporter gene Tracking gene Selection marker Vector type
pEZX-PG04** Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin Non-viral
pEZX-PG02** Gaussia luciferase (GLuc) N/A* Puromycin Non-viral
pEZX-PF02 eGFP N/A* Puromycin Non-viral
pEZX-PM02 mCherry N/A* Puromycin Non-viral
pEZX-LvPG04** Gaussia luciferase (GLuc) Secreted alkaline phosphatase (SEAP) Puromycin HIV Lentiviral
pEZX-LvPG02** Gaussia luciferase (GLuc) N/A* Puromycin HIV Lentiviral
pEZX-LvPF02 eGFP N/A* Puromycin HIV Lentiviral
pEZX-LvPM02 mCherry N/A* Puromycin HIV Lentiviral
pEZX-LvPM03 tdTomato N/A* Puromycin HIV Lentiviral

* A separate vector is available for SEAP expression. ** These Gaussia luciferase cloning vectors are available for purchase.


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