Home -> Proteomics -> Transcription Factor Activity -> Quantitative TF Activity Determination (Chemoluminescent Plate Assay)

Quantitative TF Activity Determination (Chemoluminescent Plate Assay)

• Quantify transcription factor activation
• Simpler and quicker than gel shift assay
• Multiple samples can be analyzed simultaneously

Signosis TF Filter Plate Assays are ideal for identifying transcription factor (TF) binding activity to DNA. A specific biotin-labeled TF DNA binding sequence (probe) is mixed with nuclear extracts to allow formation of TF-DNA complexes. The resulting TF-DNA complexes are bound to a filter plate, the free probe is removed in the flow-through. The bound labeled DNA probe is then eluted from the filter and collected for quantitative analysis by binding to complementary sequences immobilized on the hybridization plate. The captured DNA probe is further detected with streptavidin-HRP. Luminescence is reported as relative light units (RLUs) using a microplate luminometer.
Unlike gel shift assays, 96 samples can be analyzed in one experiment. TF Filter Plate Assays are offered for a large number of different transcription factors, see table below.

Diagram of NFkB Filter Plate Assay
Image

Related Links

TF Multiplex Assay Kits (Hybridization Plate based)
Signosis Nuclear Protein Extraction Kit

 

 

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Description Cat# Size Price    
Transcription Factor Filter Plate Assay without probe FA-0010-SO 96 reactions 492 € DETAILS   Add to Cart 
AP1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0004-SO 96 reactions 235 € DETAILS   Add to Cart 
AP1 Transcripton Factor Filter Plate Assay FA-0004-SO 96 reactions 587 € DETAILS   Add to Cart 
AP2 Probe and Plate for Transcription Factor Filter Plate Assay PP-0007-SO 96 reactions 235 € DETAILS   Add to Cart 
AP3 Probe and Plate for Transcription Factor Filter Plate Assay PP-0049-SO 96 reactions 235 € DETAILS   Add to Cart 
AP4 Probe and Plate for Transcription Factor Filter Plate Assay PP-0050-SO 96 reactions 235 € DETAILS   Add to Cart 
AR Probe and Plate for Transcription Factor Filter Plate Assay PP-0008-SO 96 reactions 235 € DETAILS   Add to Cart 
ATF2 Probe and Plate for Transcription Factor Filter Plate Assay PP-0009-SO 96 reactions 235 € DETAILS   Add to Cart 
Brn3 Probe and Plate for Transcription Factor Filter Plate Assay PP-0010-SO 96 reactions 235 € DETAILS   Add to Cart 
C/EBP Probe and Plate for Transcription Factor Filter Plate Assay PP-0011-SO 96 reactions 235 € DETAILS   Add to Cart 
CAR Probe and Plate for Transcription Factor Filter Plate Assay PP-0013-SO 96 reactions 235 € DETAILS   Add to Cart 
CBF Probe and Plate for Transcription Factor Filter Plate Assay PP-0012-SO 96 reactions 235 € DETAILS   Add to Cart 
CDP Probe and Plate for Transcription Factor Filter Plate Assay PP-0014-SO 96 reactions 235 € DETAILS   Add to Cart 
COUP-TF Probe and Plate for Transcription Factor Filter Plate Assay PP-0051-SO 96 reactions 235 € DETAILS   Add to Cart 
CREB Probe and Plate for Transcription Factor Filter Plate Assay PP-0015-SO 96 reactions 235 € DETAILS   Add to Cart 
E2F-1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0016-SO 96 reactions 235 € DETAILS   Add to Cart 
EGR Probe and Plate for Transcription Factor Filter Plate Assay PP-0017-SO 96 reactions 235 € DETAILS   Add to Cart 
ELK Probe and Plate for Transcription Factor Filter Plate Assay PP-0052-SO 96 reactions 235 € DETAILS   Add to Cart 
ER Probe and Plate for Transcription Factor Filter Plate Assay PP-0003-SO 96 reactions 235 € DETAILS   Add to Cart 
ER Transcription Factor Filter Plate Assay FA-0003-SO 96 reactions 587 € DETAILS   Add to Cart 
Ets Probe and Plate for Transcription Factor Filter Plate Assay PP-0018-SO 96 reactions 235 € DETAILS   Add to Cart 
FAST-1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0019-SO 96 reactions 235 € DETAILS   Add to Cart 
FOXA1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0053-SO 96 reactions 235 € DETAILS   Add to Cart 
FoxC1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0054-SO 96 reactions 235 € DETAILS   Add to Cart 
FOXD3 Probe and Plate for Transcription Factor Filter Plate Assay PP-0055-SO 96 reactions 235 € DETAILS   Add to Cart 
FOXG1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0056-SO 96 reactions 235 € DETAILS   Add to Cart 
FOXO1 (FKHR) Probe and Plate for Transcription Factor Filter Plate Assay PP-0057-SO 96 reactions 235 € DETAILS   Add to Cart 
FREAC-2 (FOXF2) Probe and Plate for Transcription Factor Filter Plate Assay PP-0058-SO 96 reactions 235 € DETAILS   Add to Cart 
GAS/ISRE Probe and Plate for Transcription Factor Filter Plate Assay PP-0020-SO 96 reactions 235 € DETAILS   Add to Cart 
GATA Probe and Plate for Transcription Factor Filter Plate Assay PP-0021-SO 96 reactions 235 € DETAILS   Add to Cart 
Gfi-1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0059-SO 96 reactions 235 € DETAILS   Add to Cart 
Gli-1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0060-SO 96 reactions 235 € DETAILS   Add to Cart 
GR/PR Probe and Plate for Transcription Factor Filter Plate Assay PP-0022-SO 96 reactions 235 € DETAILS   Add to Cart 
HEN(NSCL-1) Probe and Plate for Transcription Factor Filter Plate Assay PP-0061-SO 96 reactions 235 € DETAILS   Add to Cart 
HIF1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0002-SO 96 reactions 235 € DETAILS   Add to Cart 
HIF1 Transcripton Factor Filter Plate Assay FA-0002-SO 96 reactions 587 € DETAILS   Add to Cart 
HNF-1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0062-SO 96 reactions 235 € DETAILS   Add to Cart 
HNF4 Probe and Plate for Transcription Factor Filter Plate Assay PP-0023-SO 96 reactions 235 € DETAILS   Add to Cart 
HOX4C Probe and Plate for Transcription Factor Filter Plate Assay PP-0063-SO 96 reactions 235 € DETAILS   Add to Cart 
HoxA-5 Probe and Plate for Transcription Factor Filter Plate Assay PP-0064-SO 96 reactions 235 € DETAILS   Add to Cart 
HSF Probe and Plate for Transcription Factor Filter Plate Assay PP-0065-SO 96 reactions 235 € DETAILS   Add to Cart 
IRF Probe and Plate for Transcription Factor Filter Plate Assay PP-0024-SO 96 reactions 235 € DETAILS   Add to Cart 
KLF4 Probe and Plate for Transcription Factor Filter Plate Assay PP-0066-SO 96 reactions 235 € DETAILS   Add to Cart 
MEF2 Probe and Plate for Transcription Factor Filter Plate Assay PP-0025-SO 96 reactions 235 € DETAILS   Add to Cart 
Myb Probe and Plate for Transcription Factor Filter Plate Assay PP-0026-SO 96 reactions 235 € DETAILS   Add to Cart 
Myc-Max Probe and Plate for Transcription Factor Filter Plate Assay PP-0027-SO 96 reactions 235 € DETAILS   Add to Cart 
MyoD Probe and Plate for Transcription Factor Filter Plate Assay PP-0067-SO 96 reactions 235 € DETAILS   Add to Cart 
MZF Probe and Plate for Transcription Factor Filter Plate Assay PP-0068-SO 96 reactions 235 € DETAILS   Add to Cart 
NF-1 Probe and Plate for Transcription Factor Filter Plate Assay PP-0028-SO 96 reactions 235 € DETAILS   Add to Cart 
NF-E2 Probe and Plate for Transcription Factor Filter Plate Assay PP-0030-SO 96 reactions 235 € DETAILS   Add to Cart 
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