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Receptor Tyrosin Kinase Phosphorylation Profiling by Antibody Arrays

For monitoring changes of tyrosine phosophorylation in your model system
• Verify pathway activation in your cell lines
• No need to perform immunoprecipitation or Western Blot
• No special equipment required
• Minimal hands-on time: convenient, semi-quantitative, and no radioactivity
• Works reliably with wide range of cell lines and high sensitivity
• Affordable and cost effective for routine use


Protein phosphorylation plays a prominent role in cell signaling, development and growth.
The RayBio® Human RTK Phosphorylation antibody array kits are a very rapid, convenient and sensitive assay to simultaneously detect multiple protein phosphorylation and can be used to monitor activation or function of important biological pathways.


The RayBio® Receptor Tyrosine Kinase Phosphorylation Antibody Array has been designed for simultaneously identifying the relative phosphorylation levels of seventy-one different Human Receptor Tyrosine Kinases (RTKs) in cell lysates.
For a list of RTK´s represented on the array, see user manual.
After incubating antibody arrays (either membranes or glass slide subarrays) with treated and untreated cell lysate, they are washed and biotinylated anti-phosphotyrosine antibody is used to detect phosphorylated tyrosines on activated receptors. After incubation with HRP-streptavidin or Alexa Fluor 555-streptavidin, the signals are visualized by chemiluminescence or fluorescence imaging system.




References:

1. Holbro T, Hynes NE (2004). ErbB receptors: directing key signaling networks throughout life. Annu Rev Pharmacol Toxicol 44: 195–217
2. Marmor MD, Skaria KB, Yarden Y (2004). Signal transduction and oncogenesis by ErbB/HER receptors. Int J Radiat Oncol Biol Phys 58: 903–913
3. Huang RP, Huang R, Fan Y, and Lin Y (2001). A novel method for high- throughput protein profiling from conditioned media and patient’s sera.. Ana. Biochem. 294(1):55-62.
4. Huang R, Lin Y, Wang CC, J et al (2002). Connexin suppresses human glioblastoma cell growth by down-regulation of monocyte chemotactic protein 1, as discovered using protein array technology. Cancer Res. 62:2806-2812.


Related Links

User Manual RTK Phosphorylation Antibody Array (Membranes)
User Manual RTK Phosphorylation Antibody Array (Glass)

 

 

Description Cat# Size Price    
Receptor Tyrosine Kinase Phosphorylation Antibody Array (Glass Chip) (4 subarrays/slide, 71 phospho-specific antibodies/subarray) AAH-PRTK-G1-4-RB 4 Sample Kit 522 € DETAILS   Add to Cart 
Receptor Tyrosine Kinase Phosphorylation Antibody Array (Glass Chip) (4 subarrays/slide, 71 phospho-specific antibodies/subarray) AAH-PRTK-G1-8-RB 8 Sample Kit 881 € DETAILS   Add to Cart 
Receptor Tyrosine Kinase Phosphorylation Antibody Array (Membrane) (71 phospho-specific antibodies/membrane) AAH-PRTK-1-2-RB 2 Sample Kit 409 € DETAILS   Add to Cart 
Receptor Tyrosine Kinase Phosphorylation Antibody Array (Membrane) (71 phospho-specific antibodies/membrane) AAH-PRTK-1-4-RB 4 Sample Kit 557 € DETAILS   Add to Cart 
Receptor Tyrosine Kinase Phosphorylation Antibody Array (Membrane) (71 phospho-specific antibodies/membrane) AAH-PRTK-1-8-RB 8 Sample Kit 874 € DETAILS   Add to Cart 

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