Home -> Proteomics -> Protein Isolation Kits -> Inclusion Body Isolation

Inclusion Body Isolation


Obtain and solubilize inclusion bodies and purify the proteins all with 1 kit
The ProteoSpin™ Inclusion Body Isolation Micro Kit facilitates the screening of E. coli clones that express recombinant proteins in inclusion bodies.

The kit includes reagents specially formulated to achieve rapid and high-quality purification of inclusion bodies using three processes:

• Lysis of bacterial cells to release inclusion bodies in solid form
• Solubilization of purified inclusion bodies
• Purification of the recombinant protein using spin column chromatography

Downstream applications include SDS-PAGE analysis, refolding experiments, mass spectrometry, further purification and scale up.



The ProteoSpin™ Inclusion Body Isolation Micro Kit employs spin-column chromatography using Norgen’s patented protein resin as an ion exchanger. Each spin column can isolate up to 50 ug of either acidic or basic protein from 1.5 mL of bacterial culture.
Capacity per spin tube is approximately 2-50 ug and may vary depending on protein sample.
The protocol was designed for use with 1.5 mL of bacterial cultures harvested at the end of the protein induction period.
A Macro Kit for up to 12 mg protein from 100 ml bacterial culture is also available.



Image


Following gene expression, inclusion bodies were extracted, solubilized and proteins were purified. Recovered proteins were analyzed on 12% SDS-PAGE and stained with Coomassie Brilliant Blue R-250. Numbers represent kDa sizes of protein bands.








ProteoSpin™ Inclusion Body Isolation Micro Kit Contents

( A Macro Kit is also available, see below )

Each ProteoSpin™ Inclusion Body Isolation Micro Kit provides the following essential components for use with the provided protocols:

Cell Lysis reagent for inclusion body release, also available seperately - see below
Inclusion Body solubilization reagent, also available seperately - see below
• Column activation and wash buffer for acidic proteins
• pH binding buffer for acidic proteins
• Column activation and wash buffer for basic proteins
• pH binding buffer for basic proteins
• Elution buffer
• Neutralizer
• ProteoSpin™ micro spin columns
• Collection tubes (2 mL capacity)
• Final collection tubes (1.7 mL capacity)
• Syringes (1 mL capacity)
• Needles (20 gauge)
•ProteoSpin™ Inclusion Body Isolation Micro Kit application manual
• Protocol reference card for both acidic and basic procedures


Customer-supplied Reagents and Equipment:

• Benchtop microcentrifuge
• Micropipetters
• Sterile, deionized water or Milli-Q water


ProteoSpin Inclusion Body Isolation Kit Benefits

• Three kits in one for acidic and basic proteins -
The kit provides essential reagents for cell disruption, inclusion body solubilization and purification using spin column chromatography. The kit includes solutions and protocols for use with either acidic or basic proteins.

• Gentle disruption procedure provides high purity final product - Cell lysis is accomplished through non-ionic detergent chemical disruption. The use of a needle and syringe reduces viscosity and facilitates purification, producing protein often >95% pure.
Proteins are then ready for SDS-PAGE, 2D gels, mass spectrometry analysis or other procedures.

• Convenient process volume - Analyze 1.5 mL of microbial cell culture yielding 2–50 ug of protein or 100 ml bacterial cell culture to obtain up to 12 mg of protein

• Long shelf life - The kit uses an enzyme-free process with stable solutions for longer shelf life.

• Fast process time - only 60 minutes.




Applications

• Mass Spectrometry
• SDS-PAGE
• Refolding Experiments


A special inclusion body isolation and renaturation kit for GST Fusion Proteins is also available, see below(This kit is from Cell Biolabs and is using Glutathion beads as well as a special detergent buffer composition for purifying renatured GST-fusion protein)

PDF-Downloads - Will open in new browser window

User Manual for Micro Kit
Data Sheet for Micro Kit
User Manual for Macro Kit
Data Sheet for Macro Kit
Application: Time Course of Protein Expression
Application: Screening for E. coli Inclusion Bodies

 

 

Description Cat# Size Price    
Inclusion Body Solubilization Reagent 18700-NB 25 ml 77 € DETAILS   Add to Cart 
Inclusion Body Solubilization Reagent 18701-NB 100 ml 192 € DETAILS   Add to Cart 
ProteoSpin Macro Inclusion Body Isolation Kit 17700-NB 4 rxns 210 € DETAILS   Add to Cart 
ProteoSpin Micro Inclusion Body Isolation Kit 10300-NB 20 rxns 360 € DETAILS   Add to Cart 
Rapid GST Inclusion Body Solubilization and Renaturation Kit AKR-110-CB for 5 liters of bacterial culture 467 € DETAILS   Add to Cart 

BioCat Special

% Special Offers

Benefit from our current promotions

SARS-CoV-2 / COVID-19

NEW! Tools for
SARS-CoV-2
Research

Quantibody Arrays

Simultaneously quantify up to 1,000 cytokines using glass-based antibody arrays

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Bioline

Bioline Products at BioCat

Protein Extraction

Protein Extraction

Apply spin column-based Minute Protein Extraction Kits for rapid and easy protein extraction and fractionation.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!