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Membrane Protein Isolation

Minute™ Plasma Membrane Protein Isolation Kit
Invent Biotechnologies Minute™ plasma membrane protein isolation kit is composed of optimized buffers (detergent and EDTA free) and protein extraction filter cartridges with 2.0 ml collection tubes. The kit is designed to rapidly isolate native total membrane proteins (organelle membrane proteins) and native plasma membrane proteins from cultured mammalian cells or tissues. Due to the use of protein extraction filter cartridges, the membrane protein isolation is simple, easy and user friendly with high yield. Unlike many commercial membrane preparation kits that require large amount of starting cells (5 millions and up). This kit offers wide range of starting cells (1-50 millions/sample). The buffers are detergent and EDTA free. A Dounce homogenizer or a tissue blender is not needed. The procedure can be completed in less than 45 min.


Minute™ Nuclear Envelope Protein Extraction Kit
The nuclear envelope is a very complex membrane-protein system that is notoriously difficult to isolate and purify due to its connection to nuclear and cytoplasmic components. Traditional method of nuclear envelope isolation and purification requires a large amount of starting material and a lengthy and tedious procedure. Invent Biotechnologies Minute™ nuclear envelope protein extraction kit is the first commercial kit designed to rapidly isolate nuclear envelope and its associated proteins in native form without using density-gradient and ultra centrifugation. Due to the use of protein extraction filter cartridges, the nuclear envelope protein isolation is simple, easy and user friendly. The nuclear envelope proteins are significantly enriched in the final prep. Unlike traditional method that requires large amount of starting cells/tissues this kit starts with only 10-20 million cells and the buffers are detergent and EDTA free. The procedure can be completed in less than 45 min with a final yield of 10-50 µg protein/sample.

The kit can be used for cultured cells or cells isolated from tissues (Minute Single Cell Isolation Kit - Cat# SC-012-IB is recommended for obtaining single cell suspension from tissues (See link below).


Minute™ Plant Microsomal Membrane Extraction Kit
Isolation of microsomal membranes from plant tissues is a common laboratory procedure. Microsomal fraction of plant cell lysate is the focus of interest in many plant research projects. Microsomal fraction is believed to be enriched for plasma membranes, endoplasmic reticulum, Golgi apparatus, vacuolar membranes and other components of membrane system. Traditional method for microsomal fraction isolation involves so called differential pelleting protocol where a series of centrifugation steps are required to obtain various membrane fractions. Traditional protocol for microsomal fraction isolation requires large amount of starting material and employs tedious ultracentrifugation steps. MM-018 offers a simple, rapid and user friendly approach for microsomal membrane extraction using small amount of starting material (200 mg). Water soluble cytosolic proteins are removed during the procedures and water insoluble microsomal fraction, especially plasma membrane fraction, is extracted with optimized buffers in a table-top microcentrfuge. The procedure is simple, rapid and no special instrument required. Native microsomal proteins can be isolated from plant tissue in about one hour without ultracentrifugation. The protein yield is in the range of 100-200 µg/sample.


Related Links

Minute Single Cell Isolation Kit
Overview: Spin Column based Protein Extraction Kits

 

 

Description Cat# Size Price    
Minute Detergent- and EDTA-free Total and Plasma Membrane Protein Isolation and Fractionation Kit (Native Proteins obtained) SM-005-IB 50 rxn 476 € DETAILS   Add to Cart 
Minute Detergent- and EDTA-free Nuclear Envelope Membrane Protein Isolation Kit (Native Proteins obtained) NE-013-IB 50 rxn 568 € DETAILS   Add to Cart 
Minute Plant Microsomal Membrane Protein Isolation Kit (Native Proteins) MM-018-IB 50 rxn 568 € DETAILS   Add to Cart 

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