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UltraMAB Antibodies

UltraMAB™, the Ultra Specific Antibody, validated against > 10,000 human antigens
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Performance and specificity are the pre-requisites for antibodies to be used for diagnostic and therapeutic applications. To ensure the superior performance, OriGene validates every UltraMAB™ monoclonal antibody according to the scientific findings and the medical records of related diseases. Major applications of validation include WB, IHC staining with over 25 types of normal and cancer human tissues, IF/ICC, and FACS.

Some commonly used diagnostic antibodies perform well in applications but cross-react with other unrelated proteins. This cross-reactivity may potentially cause unexpected side effects and generate false diagnostic reports for clinicians.

The tools to test the mono-specificity were lacking for a long time and a challenge to the antibody market.
Our partner OriGene is proud to announce that a high density protein microarray chip has been developed for antibody specificity testing. Using the 11,088 feature chip spotted with >10,000 unique over-expressed proteins, OriGene have identified UltraMAB™, the most specific antibodies for cancer biomarkers and other important diagnostic targets.

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Under current specifications, 11,088 samples were spotted in duplicates on a single nitrocellulose slide. These include 10,380 unique overexpression lysates and 708 positive and negative control samples. Serial diluted mouse, human and rabbit IgG mixture as positive controls were spotted on three subarrays highlighted in redboxes. Subarrays highlighted in yellowboxes contain serial diluted HEK293T cell lysates as negative controls. All the subarrays contain standard positive and negative controls as shown in the figure.


Example 1: HER2 (also called ERBB2) Pathology Antibodies

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Figure 1. Specificity test on 10K protein chips with antibody 4B5 (left) and OriGene’s HER2 UltraMAB UM500036 (right):
A semi-quantitative immunohistochemical assay using anti-HER2 antibody is applied to determine HER2 protein overexpression in breast cancer tissues. The specificity of the HER2 antibody (e.g. Clone4B5) is critical because the test results will help oncologist decide a patient should receive Herceptin™ treatment. By using OriGene’s 10K high-density protein microarray, we have revealed that the antibody 4B5 is not specific to HER2 protein. As shown in the graph above, this antibody also reacts with ZSCAN1B and HER4 (ERBB4). In contrast, HER2 UltraMAB (Clone UMAB36) developed by OriGene only recognizes HER2 protein and is thus specific.


Example 2: ERCC1 IHC antibodies

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Figure 2. Specificity test on 10K protein chips with 8F1 and OriGene’s ERCC1 UltraMAB (4F9)

The excision repair cross-complementation group 1 (ERCC1) protein is an important biomarker for clinicians to predict whether certain patient populations with non-small cell lung carcinoma will respond to cisplatin chemotherapy. As such, it is critical to develop highly-specific immunohistochemistry validated monoclonal antibodies for this diagnostic test. Several publications revealed that 8F1, the most commonly used antibody clone for ERCC1, exhibits cross-reactivity to an unknown protein in ERCC1 deficient cell lines. By using OriGene’s high-density protein microarray technology, the corresponding cross-reactive binding protein for 8F1 monoclonal antibody was identified. This technology also enabled OriGene to successfully develop the most specific UltraMAB™ monoclonal antibody for ERCC1 (4F9, Clone UMAB8). This data was further confirmed by western blot analysis.

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Figure 3. Western blot validation of protein microarray chip data

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Figure 4. IHC staining of OriGene’s ERCC1 UltraMAB (clone 4F9) of a pathologically validated NSCLC FFPE tissue sections.
Related Links

Publication: ERCC1 Antibody Validation by Protein Array

 

 

Description Cat# Size Price    
Purified anti-BUB1B mouse monoclonal antibody, clone UMAB7 UM500007-OR 100 ul 468 € DETAILS   Add to Cart 
Purified anti-CD2 mouse monoclonal antibody, clone UMAB6 UM500006-OR 100 ul 468 € DETAILS   Add to Cart 
Purified anti-CK19 mouse monoclonal antibody, clone UMAB2 UM500002-OR 100 ul 468 € DETAILS   Add to Cart 
Purified anti-CK19 mouse monoclonal antibody, clone UMAB3 UM500003-OR 100 ul 468 € DETAILS   Add to Cart 
Purified anti-ERCC1 mouse monoclonal antibody, clone UMAB8 UM500008-OR 100 ul 468 € DETAILS   Add to Cart 
Purified anti-HP mouse monoclonal antibody, clone UMAB10 UM500010-OR 100 ul 468 € DETAILS   Add to Cart 
Purified anti-XRCC1 mouse monoclonal antibody, clone UMAB40 UM500037-OR 100 ul 468 € DETAILS   Add to Cart 
Purified BMP4 mouse monoclonal antibody, clone UMAB42 UM500038-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CD20 mouse monoclonal antibody, clone UMAB37 UM800001-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CD20 mouse monoclonal antibody, clone UMAB38 UM800002-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CD20 mouse monoclonal antibody, clone UMAB39 UM800003-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CD5 mouse monoclonal antibody, clone UMAB9 UM500009-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CDH2 mouse monoclonal antibody, clone UMAB23 UM500023-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CK8 mouse monoclonal antibody, clone UMAB1 UM500001-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CTNNB1 mouse monoclonal antibody, clone UMAB14 UM500014-OR 100 ul 468 € DETAILS   Add to Cart 
Purified CTNNB1 mouse monoclonal antibody, clone UMAB15 UM500015-OR 100 ul 468 € DETAILS   Add to Cart 
Purified ERBB2 / HER2 mouse monoclonal antibody, clone UMAB33 UM500033-OR 100 ul 468 € DETAILS   Add to Cart 
Purified ERBB2 / HER2 mouse monoclonal antibody, clone UMAB34 UM500034-OR 100 ul 468 € DETAILS   Add to Cart 
Purified ERBB2 / HER2 mouse monoclonal antibody, clone UMAB35 UM500035-OR 100 ul 468 € DETAILS   Add to Cart 
Purified ERBB2 / HER2 mouse monoclonal antibody, clone UMAB36 UM500036-OR 100 ul 468 € DETAILS   Add to Cart 
Purified ERCC1 mouse monoclonal antibody, clone UMAB11 UM500011-OR 100 ul 468 € DETAILS   Add to Cart 
Purified FOLH1 mouse monoclonal antibody, clone UMAB25 UM500025-OR 100 ul 468 € DETAILS   Add to Cart 
Purified FOLH1 mouse monoclonal antibody, clone UMAB26 UM500026-OR 100 ul 468 € DETAILS   Add to Cart 
Purified FOLH1 mouse monoclonal antibody, clone UMAB27 UM500027-OR 100 ul 468 € DETAILS   Add to Cart 
Purified GFAP mouse monoclonal antibody, clone UMAB5 UM500005-OR 100 ul 468 € DETAILS   Add to Cart 
Purified MEF2C mouse monoclonal antibody, clone UMAB17 UM500017-OR 100 ul 468 € DETAILS   Add to Cart 
Purified PECAM1 mouse monoclonal antibody, clone UMAB29 UM500029-OR 100 ul 468 € DETAILS   Add to Cart 
Purified PECAM1 mouse monoclonal antibody, clone UMAB30 UM500030-OR 100 ul 468 € DETAILS   Add to Cart 
Purified PECAM1 mouse monoclonal antibody, clone UMAB31 UM500031-OR 100 ul 468 € DETAILS   Add to Cart 
Purified PECAM1 mouse monoclonal antibody, clone UMAB32 UM500032-OR 100 ul 468 € DETAILS   Add to Cart 
Purified S100P mouse monoclonal antibody, clone UMAB24 UM500024-OR 100 ul 468 € DETAILS   Add to Cart 
Purified SERPINB4 mouse monoclonal antibody, clone UMAB16 UM500016-OR 100 ul 468 € DETAILS   Add to Cart 
Purified SQSTM1 mouse monoclonal antibody, clone UMAB12 UM500012-OR 100 ul 468 € DETAILS   Add to Cart 
Purified SQSTM1 mouse monoclonal antibody, clone UMAB13 UM500013-OR 100 ul 468 € DETAILS   Add to Cart 
Purified TP53 mouse monoclonal antibody, clone UMAB18 UM500018-OR 100 ul 468 € DETAILS   Add to Cart 
Purified TP53 mouse monoclonal antibody, clone UMAB19 UM500019-OR 100 ul 468 € DETAILS   Add to Cart 
Purified XPF mouse monoclonal antibody, clone UMAB20 UM500020-OR 100 ul 468 € DETAILS   Add to Cart 
Purified XPF mouse monoclonal antibody, clone UMAB21 UM500021-OR 100 ul 468 € DETAILS   Add to Cart 
Purified XPF mouse monoclonal antibody, clone UMAB22 UM500022-OR 100 ul 468 € DETAILS   Add to Cart 

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