Green Fluorescent Proteins (GFP s)
TagGFP2 Monomeric Green Fluorescent Protein
• Bright green fluorescence
• Monomeric, successful performance in fusions
• High pH- and photostability
• Fast protein maturation
• Recommended for protein labeling and FRET applications
TagGFP2 (scientific name mTagGFP) is the improved variant of TagGFP, the mutant of the Aequorea macrodactyla GFP-like protein [Xia et al., 2002, Subach et al., 2008]. TagGFP2 possesses bright green fluorescence with excitation/emission maxima at 483 and 506 nm, respectively. TagGFP2 matures 1,6-fold faster than TagGFP and is characterized by the improved performance in fusions. Compared to EGFP, TagGFP2 provides about the same brightness of fluorescence but is significantly more photostable. TagGFP2 is specially optimized for expression
Because of monomeric nature, TagGFP2 is mainly intended for protein localization studies and expression in long-term cell cultures. In FRET applications, TagGFP2 can be used as a donor for red fluorescent protein TagRFP or as an acceptor for blue fluorescent protein TagBFP.
Performance and Use of TagGFP2
TagGFP2 can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagGFP2 expression vectors give bright fluorescent signals within 10-12 hrs after transfection. No cell toxic effects and visible protein aggregation are observed.
TagGFP2 performance in fusions has been demonstrated in the beta-actin, alpha-tubulin and mitochondria-targeting signal models. It can be used in multicolor labeling applications with cyan, yellow, red, and far-red fluorescent dyes.
TagGFP2 expression in transiently transfected mammalian cells.
TurboGFP Green Fluorescent Protein
• Bright green fluorescence
• Fast maturation at a wide range of temperatures
• High pH-stability and photostability
• Proven suitability to generate stably transfected cell lines
• Destabilized variant is available
• Recommended for gene expression analysis and cell and organelle labeling
TurboGFP is an improved variant of the green fluorescent protein CopGFP cloned from copepod Pontellina plumata (Arthropoda; Crustacea; Maxillopoda; Copepoda) [Shagin et al., 2004]. It possesses bright green fluorescence (excitation/ emission max = 482/ 502 nm) that is visible earlier than fluorescence of other green fluorescent proteins.
Performance and Use of TurboGFP
TurboGFP can be expressed and detected in a wide range of organisms including cold-blooded animals. Mammalian cells transiently transfected with TurboGFP expression vectors give bright fluorescent signals in 8-10 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed. TurboGFP can be used in multicolor labeling applications with blue, true-yellow, red, and far-red fluorescent dyes.
Expression of TurboGFP and TurboGFP-tagged fusions in mammalian cells.
A - TurboGFP expression in 293T cells; B - TurboGFP-BID fusion expression in HeLa cells; C,D - TurboGFP-actin fusion expression in HeLa cells.
Xia, NS et al. (2002) Bioluminescence of Aequorea macrodactyla, a common jellyfish species in the East China Sea. Mar Biotechnol (NY). 4:155-62.
Shagin, DA et al. (2004) GFP-like proteins as ubiquitous Metazoan superfamily: evolution of functional features and structural complexity. Mol. Biol. Evol. 21:841-50.
Subach, OM et al. (2008) Conversion of Red Fluorescent Protein into a Bright Blue Probe. Chem Biol. 15:1116-24.
DMEMgfp Antibleaching Medium
TagGFP2-C vector for C-terminal fusions
TagGFP2-N vector for N-terminal fusions
Gateway TurboGFP-C vector f. C-terminal protein fusions
Gateway TurboGFP-N vector f. N-terminal protein fusions
TurboGFP-C vector for C-terminal fusions
TurboGFP-N vector for N-terminal protein fusions
TurboGFP cDNA vector
Cell Lines stably transfected with fluorescent proteins
TurboGFP-mito vector for labeling mitochondria
Cell Lines with fluorescently labeled mitochondria
Promoterless TurboGFP vector
Recombinant TurboGFP Protein