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ELISA Multiple Protein Strips Kits

Multiplex - One single ELISA strip allows the measurement of 8 Cytokines.
Flexible - Comparisons can be made between 2 to 12 samples.
Quantitative profiling - Quantitative comparison of expression among samples.
Quantitative analysis - Protein standards are available separately.
No need of capital equipment - unlike beads-based assays.
Simple procedure - all in one system with a pre-coated plate.
Wide sample type selection – Suitable for any biological liquid material and cell lysate, such as sera, cerebrospinal fluid (CSF), urine and tissue homogenate.

Human ELISA Strip Kits
In each well of the strip, a primary antibody against a specific cytokine is coated. 8 wells of the strip are coated with 8 different antibodies. Therefore, total 8 wells of a strip allow measurement of 8 different cytokines.

The test sample is allowed to react simultaneously with pairs of two antibodies, resulting in the cytokines being sandwiched between the solid phase and enzyme-linked antibodies. After incubation, the wells are washed to remove unbound-labeled antibodies. A HRP substrate, TMB, is added to result in the development of a blue color. The color development is then stopped with the addition of Stop Solution changing the color to yellow. The concentrations of the cytokines are directly proportional to the color intensity of the test sample. Absorbance is measured spectrophotometrically at 450 nm.



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