Cyan Fluorescent Protein (CFP)
• Bright cyan fluorescence
• Monomeric protein with successful performance in fusions
• Fast maturation, high pH-stability and photostability
• Proven suitability to generate stably transfected cell lines
• Recommended for protein labeling
TagCFP is a bright cyan mutant of wild-type GFP-like protein from jellyfish Aequorea macrodactyla. TagCFP possesses excitation maximum at 458 nm, and emission maximum at 480 nm. It is significantly brighter than commonly used ECFP.
TagCFP is mainly intended for protein labeling. It can also be used for cell and organelle labeling and for tracking the promoter activity.
Recommended Filter Sets and Antibodies
TagCFP can be recognized using Anti-Tag(CGY)FP antibody (Cat# AB121-EV and AB122-EV).
TagCFP can be detected using fluorescence filter sets for ECFP and the similar. Recommended Omega Optical filter sets are XF114-2 and XF130-2.
Performance and Use
TagCFP can be easily expressed and detected in a wide range of organisms. Mammalian cells transiently transfected with TagCFP expression vectors give bright fluorescent signals in 10-12 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.
TagCFP performance in fusions has been demonstrated in human cytoplasmic β-actin, α-tubulin, and mitochondria models.
TagCFP performance in protein fusions.
Mammalian cells expressing mitochondria-targeted TagCFP (A), a TagCFP fusion with ß-actin (B), and a TagCFP fusion with α-tubulin (C) were analyzed 24 hrs after transfection by fluorescence microscopy.
Xia, NS et al. (2002) Bioluminescence of Aequorea macrodactyla, a common jellyfish species in the East China Sea. Mar Biotechnol (NY). 4:155-62.