BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516
Home -> Proteomics -> Antibody Expression Vectors -> TGEX Vector Series for the rapid transient expression of antibody molecules in suspension cells

TGEX Vector Series for the rapid transient expression of antibody molecules in suspension cells

A tool for rapid transient expression of antibodies
Phage display only produces phage clones. Between them and fully recombinant antibodies expressed in stable cell lines lies a grey zone where rapid access to free, recombinant molecules without purification & detection headaches can become a challenge. The TGEX transient expression vector series, see table below, has been designed to bridge this gap.

The TGEX Vector Backbone
Image
Image
The TGEX™ backbone features a CMV promoter, the adenovirus tripartite leader sequence (TPL) (Logan 1984, Mariati 2010), a variable antibody domain leader sequence with its intron and convenient cloning sites to insert antibody variable regions. The constant regions are derived from the human IgG1 and kappa sequences. There is no selection marker and the overall small size of the vectors is optimized for transient transfection; expensive antibiotics to prepare large quantities of plasmid for transient transfections are not required. Using widely available cell lines and large scale transfection technologies (see manuals) yields of antibody between 10 mg/L and 100 mg/L in serum-free conditions are routinely achieved in the laboratory in just a few days.

Adapted to Multiple Antibody Formats
Full IgG, Fab fragments, Fc fusions, scFv-Fc fusion.
Image
The TGEX-SCblue vector is designed for the conversion of scFv-bearing phage clones into scFv-Fc fusions for antibody validation after biopanning.

References
Logan, J., & Shenk, T. (1984). Adenovirus tripartite leader sequence enhances translation of mRNAs late after infection. Proceedings of the National Academy of Sciences of the United States of America, 81(12), 3655–9.
Mariati, Ho, S. C. L., Yap, M. G. S., & Yang, Y. (2010). Evaluating post-transcriptional regulatory elements for enhancing transient gene expression levels in CHO K1 and HEK293 cells. Protein Expression and Purification, 69(1), 9–15.

 

 

Description Cat# Size Price    
TGEX-HC Human IgG1 Heavy Chain Cloning Vector MX001-ADL 10 ug 601 € DETAILS   Add to Cart 
TGEX-LC Human Kappa Light Chain Cloning Vector MX002-ADL 10 ug 601 € DETAILS   Add to Cart 
TGEX-HChis Human IgG1 Heavy Chain Cloning Vector with a C-terminal HIS tag for IMAC purification MX005-ADL 10 ug 601 € DETAILS   Add to Cart 
TGEX-FH Human IgG1 Fab Heavy Chain Fragment Cloning Vector MX004-ADL 10 ug 601 € DETAILS   Add to Cart 
TGEX-FC Human IgG1 Fc Fusion Cloning Vector MX003-ADL 10 ug 601 € DETAILS   Add to Cart 
TGEX-SCblue scFv-Fc Fusion Cloning Vector (for the transfer of scFv from any PADL phagemid vector and expression as an scFv-Fc fusion) MX006-ADL 10 ug 623 € DETAILS   Add to Cart 
TGEX-eGFP Control Vector for monitoring transfection efficiency during transient gene expression in mammalian cell suspensions culture MX012-ADL 10 ug 544 € DETAILS   Add to Cart 

BioCat Summer Special

Summer Special

10% OFF
Norgen Sample Preparation Kits

Electrophoresis

30% OFF BioCat Universal Agarose

Quantibody Arrays

Simultaneously quantify up to 1,000 cytokines using glass-based antibody arrays

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Bioline

Bioline Products at BioCat

Peptide Microarrays

Peptide Microarray

Benefit from PEPperCHIP Custom Peptide Microarrays fully tailored to your scientific needs.

TrueMAB Antibodies

TrueMab Antibodies

Use TrueMAB Monoclonal Antibodies raised against authentic protein antigens for immunoassays where a protein's native conformation is a critical parameter.

Protein Extraction

Protein Extraction

Apply spin column-based Minute Protein Extraction Kits for rapid and easy protein extraction and fractionation.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!