Sequencing Reaction Clean-Up Kit (96 well)

Product Description

For the rapid purification of sequencing extension products from reaction mixes

• Purify sequencing extension products from dye terminators, primers and other contaminants
• Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
• High recovery
• 96-well format for high throughput

This kit provides a rapid, simple and efficient procedure for the high-throughput purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.

Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.

Supporting Data

Figure 1. Sequencing Chromatogram of pDC-CG DNA. A single extension product was divided into 3 equal aliquots and was cleaned using either Norgen's Sequencing Reaction Clean-Up Kit, or one of two competitor kits. Sequencing data was then generated using Bigdye Terminator chemistry on the Applied Biosystems 3130xL DNA Sequencer. Panel A corresponds to the results when the sample was cleaned using Competitor 1, andthis chromatogram indicates low extension product yields. Panel Bcorresponds to the results when thesample was cleaned using Competitor 2, and this product exhibitedpoor dye terminator removal that introduced several blobs to the sequence. Panel C corresponds to the sample cleaned with Norgens Sequencing Reaction Clean-Up Kit, and shows good yield and efficient removal of dye terminator and contaminants as indicated from the chromatogram signals.