Recombinant Human LR3-IGF-1 Protein (MG)(Active)
| Specifications | |
|---|---|
| Molecular Weight: | 9.1 kDa |
| Source: | E.coli |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin Level: | < 1.0 EU per µg as determined by the LAL method. |
| Activity: | Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED50 for this effect is 0.2-1.5 ng/ml. |
| Format: | Lyophilized from a 0.2 μm filtered solution of 300mM HAc-NaAc, pH 6.5. |
| Additional Information | |
|---|---|
| Synonyms: | Insulin-Like Growth Factor I; IGF-I; Mechano Growth Factor; MGF; Somatomedin-C; IGF1; IBP1 |
| Organism: | Human |
| Accn. No.: | P05019 |
Sequence Information
Gly49-Ala118 (Glu51Arg)
Background
Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor proteins; which contains the N- and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids has 94% identity with mouse IGF-I and exhibits cross-species activity. IGF-1 binds IGF-IR; IGF-IIR; and the insulin receptor and plays a key role in cell cycle progression; cell proliferation and tumor progression. IGF-1 expression is regulated by growth hormone. R3 IGF-1 is an 83 amino acid analog of IGF-1 comprising the complete human IGF-1 sequence with the substitution of an Arg (R) for the Glu(E) at position three; hence R3; and a 13 amino acid extension peptide at the N terminus. R3 IGF-1 has been produced with the purpose of increasing biological activity. R3 IGF-1 is significantly more potent than human IGF-I in vitro.
Price
164,00 €
