myBaits Expert Respiratory Virus, individual module

Targeted whole genome sequencing of respiratory viral pathogens

Product Description

• 21 bait sets for common human respiratory viruses, used individually or combined
• Confirm existing variants or discover new genome variation
• Easily detect and strain-type viruses in a single assay
• Characterize tens to thousands of samples on a single sequencing run
• Save orders of magnitude in sequencing costs of heavily host-contaminated samples
• Compatible with degraded R/DNA e.g. wastewater, environmental samples, FFPE
  
  

These easy-to-use NGS kits are designed to target genomes of pathogenic respiratory viruses, for use by virologists, epidemiologists, evolutionary biologists, and public health researchers.

Unlike other manufacturers, Daicel Arbor provides full customization. Choose from our “precombined” option that includes all available viral bait modules, or build your own combination from our standalone panels that target individual viral groups. To customize your myBaits Expert Respiratory Virus kit with individual modules, please complete the Panel Selection form [xlsx] and submit with your order.

The flexible nature of myBaits® can capture novel variants, including point mutations, small or large indels, or other genomic changes. Our kits are compatible with enriching both long and short NGS library molecules, even from degraded targets.

Available Panels

The myBaits Expert Respiratory Virus panel collection is composed of 21 distinct but combinable probesets for targeted NGS of whole virus genomes. Daicel Arbor used hundreds of reference genomes for probe design to ensure that each module can retrieve a wide range of strains and variants.

* Shown in parentheses are number of reference genomes included in design for each module

Target Details

myBaits Expert Respiratory Virus Target File

Performance Data

The myBaits Expert Respiratory Virus panels are effective both individually as well as in the combined set of probes which includes all modules.

Using NGS libraries built from combinations of low copy numbers of viral genomic material embedded in backgrounds of 50 ng human RNA or DNA (see Fig.1), high enrichment rates of three to six orders of magnitude, and comprehensive genome coverage closely matching the non-enriched source were observed. 

Figure 1. Five mock samples with five different viruses (Influenza A, SARS-CoV-2, Rhinovirus, Roseola, and Measles) are enriched with their individual target modules as well as with the combined set of 21 modules, and compared with direct non-enriched shotgun sequencing.

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes.