myBaits Compass 1.2M SNP Kit

Simultaneously genotype ~1.2 million human genome SNPs

Product Description

The myBaits® Compass 1.2M SNP kit from Daicel Arbor Biosciences simultaneously genotypes ~1.2 million human genome SNPs. Compatible with a wide range of sample types – including degraded DNA samples – the Compass kit is a cost-effective, efficient solution for accurately genotyping millions of markers that are informative for forensic genetic genealogy (FGG) and other research applications. Highest-resolution FGG analysis with fifth degree or higher relationships can be achieved.

• Recovers SNPs from highly fragmented and contaminated DNA samples effectively and reliably
• Provides greater sensitivity and resolution than traditional sequencing methods, ideal for forensics
• Compatibility with Genealogical Databases
• Cost-effective and Scalable
  
  

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Panel design

The kit targets ~1.2 million human autosomal and X/Y-linked SNPs, as well as the full mitogenome, which have been selected to maximize compatibility with multiple databases and workflows commonly used for FGG analysis. The large number of targeted markers allows for robust kinship analysis, and for many samples, enables variant calling based on direct read coverage observation without the need for genomic imputation. The panel was curated to remove known ClinVar-associated SNPs (as of time of design).

Performance Data

Retrieve sites that WGS cannot access

Even for DNA samples with a low percentage of human DNA, myBaits Compass enables calling more and higher-coverage variants, compared to unenriched whole-genome sequencing (WGS) of the same libraries. We compared site retrieval rates of Compass capture and direct WGS for samples of different starting levels of endogenous human DNA. At 20 Gbp of PE150 sequencing, Compass-enriched libraries retrieved dramatically more sites at informative coverage levels than direct WGS, whether the starting library was built from 100% human DNA (“WGS-100%” / “Cap-100%”) or 10% human (“WGS-10%” / “Cap –10%”). For heavily contaminated samples, Compass enables sequencing of orders of magnitude in more sites than is possible with 20 Gbp direct sequencing. The six genomic DNA samples were commercially purchased and were associated with the following identified ethnicity/ancestry labels: (1) American Indian or Alaskan Native, (2) Arab, (3) Finnish / Italian, (4) Black / African American, (5) Hispanic/Latino, (6) Ashkenazi Jewish. Samples with 10% human DNA comprised 90% microbial DNA built from a commercial standard.

Figure 1: Performance Metrics and Validation – Enrichment vs WGS.

Have confidence in your variant calls with robust, reliable capture sequencing

myBaits Compass capture demonstrates extremely high concordance with WGS, retrieving the same variant calls with allelic bias or distortion, for a fraction of the sequencing amount required per sample. Using a diverse collection of individuals from six regional populations, we show that Compass enrichment coupled with 20 Gbp PE150 sequencing (“Cap”) achieves >99.5% concordance with calls from the same samples using deep (>15X) WGS (“Ref”). The genomic DNA samples were commercially purchased and had the following identified ethnicity/ancestry labels: (1) American Indian or Alaskan Native, (2) Arab, (3) Finnish / Italian, (4) Black / African American, (5) Hispanic/Latino, (6) Ashkenazi Jewish.

Figure 2: Performance Metrics and Validation - Concordance

myBaits kits are for research use only and are not validated for diagnostic or therapeutic purposes. 

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Contact us for assistance to get started with your myBaits Compass 1.2M SNP Kit.