Mouse C-Reactive Protein ELISA (Mouse CRP ELISA)

In this assay, the CRP present in samples reacts with the anti-CRP antibodies which have been adsorbed to the surface of polystyrene microtiter wells. After the removal of unbound proteins by washing, anti-CRP antibodies conjugated with horseradish peroxidase (HRP) are added. These enzyme-labeled antibodies form complexes with the previously bound CRP. Following another wash step, the bound enzyme-labeled antibodies are assayed by the addition of a chromogenic substrate, 3,3’,5,5’- tetramethylbenzidine (TMB). The quantity of bound enzyme-labeled antibody varies directly with the concentration of CRP in the sample tested; thus, the absorbance at 450 nm is a measure of the concentration of CRP in the test sample. The quantity of CRP in the test sample can be interpolated from the standard curve constructed from the standards and corrected for sample dilution.