AgriPrep™ Library Prep Kit with Index Set 4000, 96-well format

Up to 95% Reduction in Consumables

Product Description

Bred for NGS library prep workflow simplicity and throughput!  Powered by the one-step ExpressPlex workflow, AgriPrep is optimized for plant & animal low-pass WGS & SKIM-sequencing and goes from extracted genomic DNA to sequence-ready libraries in 100 minutes.

AgriPrep is available in bulk reagent formats and can be performed in ultra-high-throughput formats including 384-well or Echo-compatible plates.

Features and Benefits

• Simple workflow: One-step tagmentation & amplification, auto-normalization and early sample pooling
• Rapid: 100-minute workflow, 30 minutes hands-on
• Scalable: Automation-friendly with up to 3072 indexes and 384-well plate format compatibility
• Affordable: Up to 95% reduction in consumables and significant labor savings

Questions?

Connect with us today to get started with your AgriPrep NGS library preparation.

Simplest Library Prep Workflow on the Market

Don't let library prep be the bottleneck!

*based on publicly available standard protocols

Save Up to 95% on Consumables

Streamlined Protocol & 3072 Indexes = Scalability, Sustainability & Affordability

AgriPrep’s pre-plated reagents, one-step tagmentation & amplification method, early sample pooling, and sequential reagent addition provide a highly streamlined workflow that results in the use of <2 tips/sample.  When combined with the availability of 3072 indexes, AgriPrep offers unmatched scalability while minimizing environmental impact.

Table 1: Total tips required from DNA sample addition through post-PCR clean up and pooling. Figures based on publicly available standard protocols. AgriPrep figures based on seqWell’s Green Protocol.

AgriPrep Library Preparation Workflow

Powered by ExpressPlex

Recover More Data From Your Sequencing

Simply spike-in PhiRx™ to increase your index color-balancing!

PhiRx Indexed Control is included with every AgriPrep Library Prep Kit.

Kit Content

• Indexing Reaction Plate(s)
• Ready Reaction Mix Plate(s)
• MAGwise Paramagnetic Beads
• PhiRx Indexed Control

Users do not need to supply polymerase, primers, or barcodes. These are already pre-mixed into the reagents supplied.

Are you in need of bulk-dispensed product for your miniaturized reactions?

Get in touch with us: AgriPrep is available in bulk reagent formats and can be performed in ultra-high-throughput formats including 384-well or Echo-compatible plates.

Supporting Data

Auto-normalization maintains quality & provides cost efficiencies

Auto-normalization of mean insert size and read depth eliminates the need to individually quantify and dilute samples to ensure uniform coverage for all your critical samples.

Additional benefits include:

• Rapid protocol for higher laboratory throughput
• Labor and consumables cost savings
• Lower sequencing costs to meet coverage requirements for all samples
• Fewer opportunities for contamination or errors

Figure 3: A 24-plex library pool was generated from 10 – 200 ng of Barley genomic DNA and sequenced on an Illumina MiSeq™ i100 system. AgriPrep has a working range of 10 to 200 ng of DNA input, with auto-normalization of both mean insert size and read depth from 20 to 100 ng.  Mean insert size across the total working range (CV = 8.6%) and normalization range (CV = 6.9%). Read depth across the total working range (CV = 17.5%) and normalization range (CV = 10.5%)

Additional Resources

AgriPrep Master Index List (download)

XLEAP Recommended Guidelines

Any Questions Left?

Contact us for assistance to get started with the AgriPrep NGS Library Preparation.