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Viromer CRISPR Transfection Reagent for RNP Add to Cart

Cat#: TT100311-OR
Quantity: 500 rxns
Price: 325 €
Supplier: OriGene
Shipping: Blue Ice
User Manual  

Viromer CRISPR, an efficient RNP delivery reagent - no need for electroporation

Using Cas9 protein and gRNA complexes (RNP) is the preferred method due to less off-target effects and leaving no foot-print. Viromer CRISPR was developed to transfect CRISPR RNP into cells at high efficiency.

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• No need to use the cell machinery
• CRISPR components directly active upon transfection
• Skips the assembly step in the cytosol
• Active endosome escape leading to high CRISPR RNP transfection efficiency
• Better control of the Cas9 activity





Features and Benefits of Viromer CRISPR RNP Delivery Reagent

• Polymer-based
• High transfection efficiency
• Low cytotoxicity
• Gentle on cells: Low impact on cell viability and physiology
• Easily scalable, easy gRNA screening, suitable for HTS
• Adjustable amounts of delivered Cas9 and gRNA
• Rapid protein clearance
• Transient action limiting off-target cleavage
• No integration of Cas9 gene into the cell genome


RNP Transfection Data obtained with Viromer CRISPR Reagent

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Viromer CRISPR successfully delivered CRISPR RNP into C2C12 and produced 50% genome editing: NP (fluorescence labeled gRNA) was delivered by Viromer CRISPR. Images taken 24 hrs post transfection. T7E1 assay was used to analyze genome editing efficiency.

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CRISPR RNP (HPRT1 sgRNA and Cas9 Protein, Cat.# TP790148-OR) was transfected into A549 cells using Viromer CRISPR. Genome editing efficiency was analyzed with T7E1 assay.

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RFP CRISPR RNP (RFP sgRNA and Cas9 Protein, Cat.# TP790148-OR) was delivered in HEK293 RFP stable cells by Viromer CRISPR. Images were taken 5 days post transfection.

Related Links

Cas9 Protein (Cat.# TP790148-OR)
Synthetic sgRNA


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