Episomal iPSC Reprogramming System (EBNA-1 based) Add to Cart
The system consists of an optimized mixture of episomal plasmids expressing a
combination of reprogramming factors: Oct4, Sox2, Lin28, Klf4, L-Myc, p53shRNA, and miR-302/367 cluster. A GFP marker is also included for monitoring plasmid delivery and loss during real time. The plasmids are built in oriP/EBNA-1 (Epstein-Barr Nuclear Antigen-1) containing vectors. The presence of oriP/EBNA-1 leads to the replication and partition of the plasmids into daughter cells during the cell cycle, and the robust expression of the factors allows the generation of human iPS cells in a single transfection.