D-Sorbitol Colorimetric Assay Kit Add to Cart
• Simple procedure, takes ~ 35-40 minutes
• Fast and convenient
• Accurate assay for measuring sorbitol in various samples
• Useful over the range of 0.1-10 nmol of Sorbitol per sample
Sorbitol is one of the 6 carbon sugar alcohols. It is commonly used as an artificial sweetener, as a laxative and in cosmetics as a humectant and thickening agent. Sorbitol is produced naturally in a variety of fruits. It can be produced in humans in small amounts by the reduction of glucose by aldose reductase. Due to its poor ability to diffuse across the cell membrane, sorbitol can be trapped in cells and is believed to be one of the causes of damage (due to osmotic effects) in diabetes. Interestingly, sorbitol can be used as a screen for the O154:H7 strain of E. coli, since this strain is one of the few strains which cannot metabolize sorbitol.
BioVision’s Sorbitol Colorimetric Assay Kit is designed to measure sorbitol in a variety of samples such as foods, fruits, fruit juices, pharmaceuticals, cosmetics, paper and some other biological samples. In the assay, sorbitol is oxidized to fructose with the proportional development of intense color with an absorbance maximum at 560 nm. The assay is useful over the range of 0.1-10 nmol of Sorbitol per sample.
- Ana Andres-Hernando et al. (2017) Protective role of fructokinase blockade in the pathogenesis of acute kidney injury in mice. Nature Communications
- Miguel A. Lanaspa et al. (2014) Endogenous Fructose Production and Fructokinase Activation Mediate Renal Injury in Diabetic Nephropathy. JASN