TurboFP650-C Vector (Near Infrared Shifted Mutant of TurboFP635 (Katushka) Add to Cart
TurboFP650 Fluorescent Protein Vectors
When ordering 2 or more vectors encoding TurboFP650 you will receive a 50% discount on the second and subsequent TurboFP650 vectors.
Please use promotion code EV13 when placing your order.
Other TurboFP650 vectors:
|FP732-EV||TurboFP650-N Vector (Near Infrared Shifted Mutant of TurboFP635 (Katushka)||Add to Cart|
• Bright near-infrared fluorescence
• Fast maturation, high photostability
• Fluorescent signal is easily distinguished from background fluorescence
• Recommended for whole body imaging and multicolor applications
TurboFP650 (scientific name eqFP650) is a red-shifted variant of TurboFP635 (Katushka) [Shcherbo et al., 2010]. TurboFP650 is characterized by a strong bathochromic shift, with excitation and emission peaks at 592 nm and 650 nm, respectively. It is currently the brightest fluorescent protein with emission maxima above 635 nm.
TurboFP650 demonstrates fast maturation at 37°C and a high pH-stability and photostability. The protein does not show residual short wavelength fluorescence of intermediate or alternative chromophore forms, in contrast to E2-Crimson, which exhibits a second bright blue emission peak, and mNeptune, which has a pronounced green peak.
TurboFP650 is specially recommended for whole body imaging and multicolor applications.
Performance and Use
TurboFP650 can be easily visualized within living tissues. Mammalian cells transiently transfected with TurboFP650 expression vectors give bright fluorescent signals in 14 hrs after transfection. No cytotoxic effects or visible protein aggregation are observed.
Superior performance of TurboFP650 in whole-body imaging was demonstrated using mouse xenograft model. HEK 293T cells transiently transfected with a plasmids encoding either TurboFP635, TurboFP650, NirFP, mNeptune or E2-Crimson were injected intramuscularly into the gluteal region of mice. The cells were co-transfected with firefly luciferase plasmid to normalize the transfection efficiency and total numbers of injected cells. Imaging of cell implants and quantification at various emission wavelengths showed higher fluorescence from TurboFP650 at two excitation wavelengths.
Despite its dimeric structure, TurboFP650 can be used in some fusions. However, for protein labeling applications we recommend using specially optimized monomeric TagFPs.
TurboFP650 can be used in multicolor labeling applications with blue, cyan, green, yellow, and red (orange) fluorescent dyes.
TurboFP650 can be recognized using Anti-tRFP antibody (Cat# AB233-EV and AB234-EV).
Whole-mouse imaging with IVIS Spectrum system (Caliper).
(A) Representative fluorescence reflectance images (excitation filter, 605/30 nm and emission filter, 660/20 nm) of mice injected intramuscularly with HEK 293T cells expressing E2-Crimson, mNeptune or TurboFP650. Green asterisk denotes background fluorescence in mice injected with E2-Crimson cells. The color bar indicates radiant efficiency ×10-6; minimum is 0.001, and maximum is 0.006.
(B,C) Fluorescence efficiency from cell implants imaged with 570/30 nm (B) or 605/30 nm (C) excitation filters and various emission filters, normalized to photons from firefly luciferase to control for transfection efficiency and numbers of implanted cells. Means ± s.e.m. are shown (n = 6-10 per point). *P < 0.05 (Student´s t-test) for TurboFP650 relative to other proteins. Red line - TurboFP650, green line - mNeptune, blue line - E2-Crimson. Images and data from Shcherbo et al., 2010.
- Shcherbo, D et al (2010) Near-infrared fluorescent proteins Nat Methods [Epub ahead of print] / pmid: 20818379.