EVeryRNA cDNA Synthesis & Pre-Amplification Kit Add to Cart

Cat#: EVery200A-1-SBI
Quantity: 20 preps
Price: 357 €
Supplier: System Biosciences
Shipping: Dry Ice
User Manual  

• Get quick (<2 hrs) and easy cDNA synthesis
• Optimized for use with the EVeryRNA EV RNA Purification System
• Find low abundance RNAs with the optional pre-amplification step
• Use with most downstream applications such as PCR, qPCR and miRNA profiling
Bundle with the EVeryRNA EV Purification System available for added convenience

Accelerate your EV biomarker studies
Optimized for use with the EVeryRNA EV Purification System, the EVeryRNA cDNA Synthesis & Pre-amplification Kit provides high quality exosomal cDNA for your downstream applications such as PCR, qPCR, miRNA profiling, and RNA-seq. The kit generates cDNA through poly(A) tailing of exoRNA followed by reverse transcription with MMLV Reverse Transcriptase. This method uses the template-switching oligonucleotide property of MMLV RT to generate cDNA molecules with two adaptor primers from both 5’- and 3’- ends, which can then be amplified with the included DNA Polymerase, if desired.


Demonstration of the excellent RNA yields and robust cDNA synthesis obtained with EVeryRNA

Image
Fig.1.
EVs were isolated from 250 µL of serum using SmartSEC Single, spiked in 0.1 pmol of Cel-miR-39, and used both the EVeryRNA EV Purification System and a phenol-based kit to isolate RNA. The isolated RNA was reverse transcribed using the EVeryRNA cDNA Synthesis & Pre-amplification Kit and the copy number of Cel-miR-39 measured. The EVeryRNA EV Purification System delivered similar levels of Cel-miR-39 as the phenol-based method.

Related Links

Exosomal RNA Purification Kits


BioCat Summer Special

% Special Offers

Benefit from our current promotions

SARS-CoV-2 / COVID-19

NEW! Tools for
SARS-CoV-2
Research

Electrophoresis

30% OFF BioCat Universal Agarose

DNA and RNA Purification

Bioline

Use ISOLATE II Nucleic Acid Isolation Kits for the purification of high-quality DNA and RNA.

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!