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Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit
Specifications | |
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Research Area: | Lipid Metabolism |
Assay Category: | Fatty Acids Related Assays |
Sample Type: | serum, plasma, animal tissue, cell |
Detection Method: | Microplate reader |
Sensitivity: | 0.14 mmol/L |
Detection Range: | 0.14-4 mmol/L |
Product Information
Free fatty acids (NEFA/FFA) generate acyl-CoA under the action of acyl synthase, then acyl-CoA generates hydrogen peroxide under the oxidation of acyl oxidase. Hydrogen peroxide reacts in the presence of enzymes and chromogens, producing a red substance with maximum absorption at 546 nm. The amount of this red product is proportional to the content of free fatty acids.
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