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Aspartate Aminotransferase (AST/GOT) Activity Fluorometric Assay Kit
| Specifications | |
|---|---|
| Research Area: | Cell Metabolism |
| Assay Category: | Metabolic Enzymes |
| Sample Type: | cell, animal tissue, serum (plasma), other liquid samples |
| Detection Method: | Fluorescence microplate reader |
| Sensitivity: | 0.03 U/L |
| Detection Range: | 0.03-1.5 U/L |
Product Information
AST can catalyze the substrate to produce pyruvate. Pyruvate is oxidized to produce hydrogen peroxide, which causes the fluorescent probe to produce fluoresce. The activity of AST can be calculated by measuring the increase of fluorescence value at the excitation wavelength of 535 nm and the emission wavelength of 587 nm.
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