Exosome Characterization by NanoSight Service (NTA Service, Nanoparticle Tracking Analysis Service) Add to Cart
|Quantity:||Nanosight Data and Analysis|
• Understand the quality of your exosome preparations
• Leverage SBI’s long-term experience working with exosomes
• Send us your biofluid or exosome prep and receive data in about 3 weeks
SBI uses NTA measurements that rely on light scattering to extract particle size and the number of particles in a sample (Fig.1.). Briefly, a laser is directed at the sample and the particles in the sample scatter the light, which is imaged by a camera attached to a microscope and operating at 30 frames per second. The NTA software collects data on multiple particles simultaneously, and calculates the hydrodynamic diameter of each particle using the Stokes-Einstein equation.
Fig.1. All particles unlabeled are equally visible to NTA
Fig.2. Exosomes moving in Brownian motion and the laser light scattering is recorded and each particle tracked
How the service works
Send your biofluid samples (serum, urine, media, CSF, etc.) to BioCat - our partner
SBI will isolate and characterize exosomes. Or you can also send exosomes you have already isolated. SBI will perform the nanoparticle tracking analysis. Each sample will be gently vortexed at 2.5k for 10 sec and then bath sonicated for 10 min at 33°C to ensure adequate exosome dispersion in the solution. NanoSight measurements are carried out in 0.02 um filtered PBS to remove any background and then visualized on an LM10 NanoSight instrument.
SBI provides the particle analysis data, measured in triplicate in reports that show the mean, mode diameter size of the exosomes as well as concentration of the exosomes. Each sample is visualized independently and a video recorded of the data collection. This data video is also supplied as a .wmv file included in the service.
Additional costs for shipping your samples to our service partner might apply – please contact us for a quote.