NGS of DNA from Genetic Screen, up to 50 M Reads (screening done with Cellecta or GeCKO Library) ACADEMIC Add to Cart

Quantity: per DNA sample
Price: 1645 €
Supplier: Cellecta
Please contact us for further information.

HT Sequencing and Analysis

Cellecta pooled lentiviral libraries are provided with a complete protocol and all sequencing information to enable researchers to perform high throughput genetics screens and analysis.

For laboratories needing support for this analysis, we also provide Cellecta´s Next-Gen Sequencing and Analysis Service.

You provide BioCat with genomic DNA obtained from your cells after screening for each time point or treatment condition, Cellecta does the rest:

• Amplifies shRNA-specific barcodes* or gRNA sequences
• Performs HT sequencing on the Illumina HiSeq
• Deconvolutes sequencing data / Enumerates Barcodes or gRNA from raw sequencing data
• Provides a HT Sequencing Service Report with the relative abundance of each shRNA or gRNA sequence in each population; you receive an excel file with enumeration data (counts) of each shRNA or gRNA

Additional costs for shipping your samples to our service partner might apply - Please inquire for a quotation (see link below).

For multiple samples, bulk pricing is available, please ask for a quotation.

Other available services (please ask for a quotation):

• HT Bar-Code Sequencing of DNA (>100 M Reads)
• HT Bar-Code Sequencing of Cell Pellets (>20 M Reads)
• HT Bar-Code Sequencing of Cell Pellets (>100 M Reads)
• HT Bar-Code Sequencing of Tumors (>20 M Reads)
• HT Bar-Code Sequencing of Tumors (>100 M Reads)

• NGS Service Report
• Additional bioinformatics and pathway analysis

* Cellecta shRNA libraries contain barcodes compatible with the Illumina High-Throughput (HT) Sequencing platform and provide accurate reads. Cellecta gRNA libraries do not contain barcodes, as gRNA sequences are less complex and can be sequenced directly.

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