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Swift Amplicon 16S+ITS Panel Add to Cart

Cat#: AL-51648-SWI 
Quantity: 48 rxns
Price: 1287 €
Supplier: Swift Biosciences
Shipping: Dry Ice
User Manual  

Comprehensive, solves 16S sequencing issues, and saves costs.
Swift Amplicon 16S+ITS is a microbial identification panel which targets the 16S rRNA gene (V1-V9) and ITS in a single primer pool.

• Covers all variable regions of 16S for bacteria and archaea
• Also covers ITS1 and ITS2 for fungi
• Unique single pool multiplex PCR chemistry
• Solves sequencing complexity issues
• Saves cost and shortens turnaround time
• No PhiX or phased primers required
• Allows 2x150 reads
• Short, 2-hour workflow

Swift Amplicon Panels enable the preparation of high quality targeted next-generation sequencing (NGS) libraries from a variety of sample types. Adapters are included for dual indexing and multiplexing up to 96 samples on a sequencing run. The single-tube workflow from DNA to library can be completed within two hours.

Workflow
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Applications
• Metagenomics: Bacteria, Archaea, Fungi
• Environmental: Air, Water, Wastewater
• Agriculture: Plant microbiome, Soil – ITS for fungal
• Microbiome: Human – skin, GI tract, stool, vagina, Plant – surface, roots and soil
• Forensics: Microbiome to establish time of death
• Detection and characterization of bacterial and fungal structure in complex samples

The kit provides a single primer pool covering all the variable regions of the 16S rRNA
gene (V1-V9) and the Fungal ITS1 and ITS 2 genes, library preparation reagents, and
Illumina®-compatible adapters.

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Figure 1. The Swift Amplicon 16S+ITS panel generates libraries covering all variable regions of 16S and fungal ITS1/ITS2 targets, unlike conventional 16S rRNA gene assays that target only V3-V4, or region-specific single-plex primers that require phased reverse primers and result in low complexity libraries with limited sensitivity and low-quality sequencing.


Provides Superior Representation of a Diverse Microbial Community
Versus V3-V4 Only


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Figure 2. The Swift Amplicon 16S+ITS Panel covering all V1-V9 regions provides sensitive detection and accurate representation of each species in the sample compared to standard methods interrogating the V3-V4 region alone. Organisms marked in red were underrepresented by V3-V4 only method. Input DNA was a mix of 20 bacterial species (ATCC MSA-1003) tested with the Swift Amplicon 16S+ITS Panel, sequenced with Illumina® MiSeq® V3 (2x300bp reads).


Consistent Performance with Varying DNA Input, Sample Type,
and Read Length


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Figure 3. Using the same protocol and cycling conditions, input quantities of 10 pg to 50 ng with both ATCC MSA-1003 (bottom) and swine manure (top) gave consistent and expected sequencing results in terms of sensitivity and relative abundance.



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Figure 4. Comparison of 2x150 and 2x300 PE reads. The Swift 16S+ITS Panel identified a comparable number of species from swine manure samples.


Specifications
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