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Plasma/Serum Circulating and Exosomal RNA and DNA Purification Kit (Slurry Format) Maxi, 2 - 5ml sample input Add to Cart

Cat#: 53400-NB
Quantity: 25 preps
Price: 580 €
Supplier: Norgen
Shipping: RT
User Manual  

For rapid and simple isolation of circulating Nucleic Acid from plasma/serum samples

• Isolate all sizes of circulating and exosomal RNA and circulating DNA in one elution
• Fast and easy processing - Rapid slurry format allows for the processing of multiple samples in under 40 minutes.
• Versatile plasma and serum input volumes (2 mL to 5 mL)
• Concentrate circulating nucleic acid into small elution volumes
• Isolate inhibitor-free circulating nucleic acid

Norgen´s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides a fast, reliable and simple procedure for isolating circulating Nucleic Acid (mRNA, miRNA, exosomal RNA, Viral RNA, DNA and Viral DNA) from various amounts of plasma/serum ranging from 2 mL to 5 mL. Free-circulating nucleic acids in plasma and serum have the potential to provide biomarkers for certain cancers and disease states, and include tumor-specific extracellular nucleic acid fragments and fetal DNA in maternal blood. Free-circulating DNA is usually present as short fragments of less than 1000 bp, whereas free-circulating RNA and exosomal RNA in plasma and serum are usually present as short fragments of less than 1000 nt. In addition, plasma or serum also contains free-circulating miRNA that are as small as 21 nt. Furthermore, circulating viral DNA or viral RNA may be present in the plasma or the serum of viral-infected patients. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an efficient method for the purification of all sizes of these fragmented free-circulating Nucleic Acid from human plasma or Serum.

Purification is based on the use of Norgen’s proprietary resin as the separation matrix. The kit is able to isolate all sizes of circulating RNA, including microRNA, as well as all sizes of exosomal RNA and circulating DNA. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating nucleic acid from large sample volumes. Free-Circulating nucleic acid can be isolated from either fresh or frozen samples. The kit allows for the isolation of nucleic acid that is present in low concentrations (1-100ng/mL in human plasma). Typical yields of free-circulating nucleic acid will vary depending on the input sample, as the amount of nucleic acid present in plasma and serum will depend upon the health status of the individual and the level of nucleases present in the blood. This kit is suitable for the purification of nucleic acid from serum or plasma prepared from blood collected on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used, as heparin can significantly interfere with many downstream applications, such as RT-PCR.

Purified nucleic acid can be used in a number of sensitive downstream applications including PCR, qPCR, reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, methylation-sensitive PCR and Southern Blot Analysis.


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Isolation and Detection of Circulating RNA from Different Plasma Volumes. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit (Slurry Format) was used to isolate circulating RNA from 2mL, 3mL, 4mL and 5mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume.


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Effective Isolation of Plasma Circulating RNA from Different Volumes. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit (Slurry Format) was used to isolate circulating RNA from 2mL, 3mL, 4mL and 5mL plasma. Three microlitres of the purified RNA was then used as the template in RT-qPCR reactions to detect the human 5S gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA showed an increasing amount of RNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume.


Image
Isolation and Detection of Circulating DNA from Different Plasma Volumes. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit (Slurry Format) was used to isolate circulating DNA from 2mL, 3mL, 4mL and 5mL plasma. Three microlitres of the purified DNA was then used as the template in qPCR reactions to detect the human 5S rRNA gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA gene showed an increasing amount of DNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume.


Image
Effective Isolation of Plasma Circulating DNA from Different Volumes. Norgen’s Plasma/Serum Circulating Nucleic Acid Purification Maxi Kit (Slurry Format) was used to isolate circulating DNA from 2mL, 3mL, 4mL and 5mL plasma. Three microlitres of the purified DNA was then used as the template in qPCR reactions to detect the human 5S rRNA gene. The 5S housekeeping gene was detected from all plasma sample volumes used. The amplification of the 5S rRNA gene showed an increasing amount of DNA with increasing the sample input volume. This is represented by the decrease of the Ct value with increasing the sample input volume.

Related Links

Overview Circulating DNA Isolation Kits
Overview plasma/serum sample preparation kits


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