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RNA/DNA Purification Kit Add to Cart

Cat#: 48700-NB
Quantity: 50 preps
Price: 555 €
Supplier: Norgen
Shipping: RT
User Manual  

For sequential isolation of total RNA and genomic DNA from the same sample

• Rapid spin column procedure - Isolate total RNA and genomic DNA from a single sample in less than 30 minutes
• Isolate from small samples as well as hard-to-extract samples
• Sequential isolation of high quality RNA and DNA from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures
• Additional Proteinase K provided for hard-to-extract fibrous tissues
• Ideal for small or difficult to obtain samples
• No need to split the lysate, or to use phenol or precipitation methods
• Reduce variability - no splitting of the lysate, thus reducing inconsistent results and variability
• Isolate a diversity of RNA species, from large mRNA down to microRNA
• Complete column purification

Norgen’s RNA/DNA Purification Kit provides a rapid method for the isolation and purification of total RNA and genomic DNA sequentially from a single sample of cultured animal cells, tissue samples (particularly hard-to-extract fibrous tissues), blood, bacteria, yeast, fungi or plants. The total RNA and genomic DNA are all column purified in less than 30 minutes. This kit is ideal for researchers who are interested in studying the genome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA Purification Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA and DNA are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.

Workflow
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Recovery of True Total RNA including microRNA from Hamster Heart. Panel A is a 1X MOPS 1% agarose gel showing the RNA that was isolated from 2 different samples of 10 mg hamster heart using Norgen’s RNA/DNA Purification Kit. Norgen’s RNA/DNA Purification Kit isolated large RNA (represented by 28S and 18S rRNA) with high integrity. Moreover, it provided the added benefit of recovering small RNA without any additional protocol. Panel B is a result from a bioanalyzer resolution of the eluted RNA. Similar to the agarose gel, Norgen’s RNA/DNA Purification Kit showed the added benefit of recovering small RNA. The effectiveness in small RNA recovery was also demonstrated by gene-specific RT-qPCR. One microgram of RNA was used in RT-qPCR reactions for human S15 (for Large RNA) and miR-21 (for microRNA) genes. The RNA isolated by Norgen’s RNA/DNA Purification Kit showed detection of both small RNA (Panel C) and the large RNA (Panel D).

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Recovery of Intact, High Quality Genomic DNA from Hamster Heart. Panel A is a 1% agarose gel showing the gDNA isolated from the same hamster heart samples above using Norgen’s RNA/DNA Purification Kit. Lane M is Norgen’s HighRanger 1 kb DNA Ladder and the sample lanes contain 10 µL of each of the 100 µL elutions. The gel showed high quality, and intact genomic DNA. Panel B is the result of qPCR amplification of 25 ng of eluted genomic DNA using 5S rRNA-specific primers. Genomic DNA isolated using Norgen’s RNA/DNA Purification Kit is of high quality with effective qPCR amplification.


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