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CleanAll RNA and DNA Clean-Up and Concentration Micro Kit Add to Cart

Cat#: 23800-NB
Quantity: 50 preps
Price: 303 €
Supplier: Norgen
Shipping: RT
User Manual  

For the rapid and efficient purification, cleanup and concentration of RNA or DNA

• Purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA)
• RNA is preferentially purified from other reaction components such as proteins and nucleotides, without the use of phenol or chloroform
• Purifies all sizes of DNA, from small PCR products to plasmids to genomic DNA.
• Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 20 minutes.
• Versatile performance - Both DNA and RNA can be cleaned and concentrated using the same kit. Clean-up nucleic acids from a variety of isolation methods and upstream enzymatic reactions.
• Optional endotoxin removal - Optional protocol is provided for the rapid removal of endotoxins from previously purified RNA or DNA.

Norgen’s CleanAll RNA/DNA Clean-Up and Concentration Micro Kit provides a rapid method for the purification, cleanup and concentration of RNA or DNA from different isolation methods or upstream applications. The kit can be used as an alternative to organic extraction and ethanol precipitation to clean up various enzymatic reactions. The CleanAll Kit purifies RNA from phenol/guanidine-based protocols or from various upstream enzymatic reactions such as DNase treatment, labeling and in vitro transcription. Furthermore, endotoxins can be removed from previously purified RNA solutions. The kit can be used to clean up DNA from digestions, ligations, PCR reactions, labeling reactions, DNA modification reactions and staining. The kit also provides a protocol for the rapid removal of endotoxins from previously purified DNA down to 0.1 EU/µg DNA or less.

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the samples or reaction containing RNA or DNA is mixed with Binding Solution and the nucleic acids are bound to Norgen´s column (BIND). Under these conditions only the nucleic acids will bind to Norgen´s resin while most of the contaminating proteins and nucleotides are removed in the flowthrough. The bound nucleic acid is then washed to remove any remaining impurities (WASH). Lastly, the purified total RNA or DNA is eluted into the provided Elution Buffer L or water (ELUTE). Please see the procedure flowchart below.

Workflow
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High Recoveries and Efficient Endotoxin Removal. High recoveries of DNA can be seen when using the CleanAll kit, and these high recoveries are not compromised even during endotoxin removal. In Panel A, 1 µg of a PCR product was cleaned using the CleanAll RNA/DNA Clean-Up and Concentration Kit as well as Norgen´s PCR Purification Kit. More than 0.9 µg of the DNA was recovered from both kits with a comparable percent recovery of over 90%. In Panel B, 10 µg of plasmid DNA preparation was cleaned from endotoxin by using the CleanAll RNA/DNA Kit. Endotoxin levels of the plasmid DNA sample were reduced from 0.75 EU/µg plasmid DNA to less 0.05 EU/µg plasmid DNA. Approximately 95% of the endotoxin units in the DNA sample were removed using the kit, therefore the kit allows for high recoveries coupled with exceptional cleanup.


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Clean-Up of RNA with High Recovery. Norgen’s CleanAll RNA/DNA Clean-Up and Concentration Kit can be used to clean up various enzymatic reactions including DNase treatment. In Figure 2 above, Lane 1 is the RNA input, while lanes 2-5 contain the RNA that has been cleaned using Norgen’s CleanAll Kit. It can be seen that in all cases the recovery is high, and the purified RNA is intact and of a high quality.


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