Accel-NGS 2S PCR-Free DNA Library Kit for Illumina Add to Cart

Cat#: 20024-SWI
Quantity: 24 rxns
Price: 754 €
Supplier: Swift Biosciences
Shipping: Dry Ice

User Manual  

PCR-Free NGS Prep with Low Input Capability

Eliminate Sequencing Bias Introduced by Polymerase

• PCR-free libraries from inputs as low as 10 ng
• Sequential repair steps for damaged samples
• Compatiblity with low input cfDNA and FFPE samples
• Exceptional library prep efficiency and library complexity for deep sequencing
• Proprietary adapter attachment chemistry minimizes bias and supports low input
• Excellent evenness of coverage, balanced coverage of AT-/GC-rich regions
• Readily automatable

The Accel-NGS 2S PCR-free DNA Library Kit for Illumina platforms enables preparation of high complexity NGS libraries from double-stranded DNA. The kit is designed with two types of users in mind: those who have sufficient starting material to produce libraries free of bias from polymerase and those wishing to use a polymerase of choice for amplification. PCR-free libraries may be generated from as little as 100 ng of high quality genomic DNA or 10 ng of circulating cell-free DNA (cfDNA). PCR primers are included for users with low inputs (10 pg to 100 ng) who wish to use a polymerase of choice.

The kit is also available with polymerase for as low as 10 pg input DNA.

Applications

• Whole Genome Sequencing (WGS), including WGA samples
• Cell-free DNA (cfDNA) Sequencing
• ChIP-Seq
• Metagenomics

The kit utilizes Illumina-compatible adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.



Accel-NGS 2S PCR-Free DNA Library Kit Workflow:

Each step is color-coded to make the protocol easy to follow.

Image

Clean-up beads are not provided with the kit, we recommend the SPRIselect™ Reagent Kit from Beckman Coulter.

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