Accel-NGS 2S PCR-Free DNA Library Kit for Illumina - 24 reactions Add to Cart
An Indexing Kit is required for complete functionality. Please select:
PCR-Free NGS Prep with Low Input Capability
Eliminate Sequencing Bias Introduced by Polymerase
• PCR-free libraries from inputs as low as 10 ng
• Sequential repair steps for damaged samples
• Compatiblity with low input cfDNA and FFPE samples
• Exceptional library prep efficiency and library complexity for deep sequencing
• Proprietary adapter attachment chemistry minimizes bias and supports low input
• Excellent evenness of coverage, balanced coverage of AT-/GC-rich regions
• Readily automatable
The Accel-NGS 2S PCR-free DNA Library Kit for Illumina platforms enables preparation of high complexity NGS libraries from double-stranded DNA. The kit is designed with two types of users in mind: those who have sufficient starting material to produce libraries free of bias from polymerase and those wishing to use a polymerase of choice for amplification. PCR-free libraries may be generated from as little as 100 ng of high quality genomic DNA or 10 ng of circulating cell-free DNA (cfDNA). PCR primers are included for users with low inputs (10 pg to 100 ng) who wish to use a polymerase of choice.
The kit is also available with polymerase for as low as 10 pg input DNA.
• Whole Genome Sequencing (WGS), including WGA samples
• Cell-free DNA (cfDNA) Sequencing
The kit utilizes Illumina-compatible adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.
Accel-NGS 2S PCR-Free DNA Library Kit Workflow:
Each step is color-coded to make the protocol easy to follow.
Clean-up beads are not provided with the kit, we recommend the SPRIselect™ Reagent Kit from Beckman Coulter.
- Bolognesi C et al. (2016) Digital Sorting of Pure Cell Populations Enables Unambiguous Genetic Analysis of Heterogeneous Formalin-Fixed Paraffin-Embedded Tumors by Next Generation Sequencing. Scientific Reports 6:20944. doi:10.1038/srep20944
- Sundaram, AYM et al. (2016) A comparative study of ChIP-seq sequencing library preparation methods. BMC Genomics 17:816.