BioCat GmbH • Im Neuenheimer Feld 584 • D-69120 Heidelberg • Tel.: +49(0)6221 7141516

Accel-NGS 2S PCR-Free Library Kit for Illumina - 24 reactions Add to Cart

Cat#: 20024-SWI 
Quantity: 24 rxns
Price: 723 €
Supplier: Swift Biosciences
Shipping: Dry Ice

User Manual  

PCR-Free NGS Prep with Low Input Capability

Eliminate Sequencing Bias Introduced by Polymerase

• PCR-free libraries from inputs as low as 10 ng
• Sequential repair steps for damaged samples
• Compatiblity with low input cfDNA and FFPE samples
• Exceptional library prep efficiency and library complexity for deep sequencing
• Proprietary adapter attachment chemistry minimizes bias and supports low input
• Excellent evenness of coverage, balanced coverage of AT-/GC-rich regions
• Readily automatable

The Accel-NGS 2S PCR-free DNA Library Kit for Illumina platforms enables preparation of high complexity NGS libraries from double-stranded DNA. The kit is designed with two types of users in mind: those who have sufficient starting material to produce libraries free of bias from polymerase and those wishing to use a polymerase of choice for amplification. PCR-free libraries may be generated from as little as 100 ng of high quality genomic DNA or 10 ng of circulating cell-free DNA (cfDNA). PCR primers are included for users with low inputs (10 pg to 100 ng) who wish to use a polymerase of choice.

The kit is also available with polymerase for as low as 10 pg input DNA.

Go to product line overview to choose the library prep and indexing adapter kits for your application. For complete functionality of the kit an indexing kit is always required.

Applications

• Whole Genome Sequencing (WGS), including WGA samples
• Cell-free DNA (cfDNA) Sequencing
• ChIP-Seq
• Metagenomics

The kit utilizes Illumina-compatible adapter sequences and has been validated on the MiSeq® and HiSeq® platforms.



Accel-NGS 2S PCR-Free DNA Library Kit Workflow:

Each step is color-coded to make the protocol easy to follow.

Image

Clean-up beads are not provided with the kit, we recommend the SPRIselect™ Reagent Kit from Beckman Coulter.

Related Links

Product Line Overview and Decision Tree
Product Information Sheet



DNA and RNA Purification

Bioline

Use ISOLATE II Nucleic Acid Isolation Kits for the purification of high-quality DNA and RNA.

BioCat Spring Special

Spring Special

10% OFF
3'UTR microRNA Target & Promoter Analysis

Email Newsletter

Subscribe to the BioCat Email Newsletter.

Clone Resources

BioCat Clone Resources

Browse two of the most renowned clone resources of full-length cDNA, ORF, and shRNA clones as well as siRNA and yeast knockout strains.

Genome Engineering

Genome Engineering

Use the CRISPR/Cas9 SmartNuclease System to edit the genome.

ExoQuick and ExoQuick-TC

ExoQuick Exosome Isolation

Benefit from the most cited exosome isolation reagent for efficient exosome isolation and exosomal RNA purification from biofluids or culture media.

Home
Imprint / Impressum | Privacy Policy / Datenschutzerklärung
Top of Page Up!