RNA/DNA/Protein Purification Plus Kit Add to Cart
Special570.60 € - valid until 30 September 2019
For sequential isolation of total RNA, genomic DNA and total proteins from the same sample
• Rapid procedure - Isolate total RNA, genomic DNA and total proteins from a single sample in less than 30 minutes
• Sequentially isolate and purify total RNA, genomic DNA and total proteins from a single sample
• Isolate a diversity of RNA species, from large mRNA down to microRNA
• No phenol extractions
• Isolate high quality RNA, DNA and proteins
• Isolate from small samples - Sequential isolation of RNA, DNA and protein from a single sample. Ideal for precious, difficult to obtain or small samples such as biopsy material or single foci from cell cultures.
• Reduce variability - no splitting of the lysate, thus reducing inconsistent results and variability
• Purify RNA/DNA/Protein from cultured animal cells, tissues, blood, bacteria, yeast, fungi or plants
• Complete column purification - The RNA, DNA and proteins are all column purified
• Process higher input amounts than with Norgen´s RNA/DNA/Protein Purification Kit (Cat# 23500)
Norgen’s RNA/DNA/Protein Purification Plus Kit provides a rapid method for the isolation and purification of total RNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. The total RNA, genomic DNA and proteins are all column purified in less than 30 minutes (Figure 1). This kit is ideal for researchers who are interested in studying the genome, proteome and transcriptome of a single sample, such as for studies of microRNA profiling, gene expression including gene silencing experiments or mRNA knockdowns, studies involving biomarker discovery, and for characterization of cultured cell lines. Norgen’s RNA/DNA/Protein Purification Plus Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as biopsy materials or single foci from cell cultures, as it eliminates the need to fractionate the sample. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample, thereby eliminating inconsistent results. The purified macromolecules are of the highest purity and can be used in a number of different downstream applications.
Purification is based on spin column chromatography. Briefly, the process involves first lysing the cells or tissue of interest with the provided Lysis Solution and the lysate is loaded onto a DNA Purification Column. Under these conditions only the DNA will bind to the column while the RNA and proteins are removed in the flowthrough (BIND 1). Next, the bound DNA is washed with the to remove impurities (WASH 1), and the purified DNA is eluted with the DNA Elution Solution (ELUTE 1). The flowthrough of the DNA purification step is then loaded onto a RNA/Protein Purification Column. Total RNA, including microRNAs, will bind to the column while the proteins are removed in the flowthrough (BIND 2). . The bound RNA is then washed (WASH 2), and eluted from the column (ELUTE 2). The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA).
The proteins that are present from the RNA binding flowthrough can now be loaded directly onto an SDS-PAGE gel for visual analysis. Alternatively, the protein samples can be further purified using the same RNA/Protein Purification Column. The RNA flowthrough is pH adjusted and loaded back onto the column in order to bind the proteins that are present (BIND 3). The bound proteins are washed with the provided wash buffer (WASH 3), and are then eluted such that they can be used in downstream applications (ELUTE 3).
The purified RNA, DNA and proteins are of the highest integrity and can be used in a number of downstream applications.
- Sarah Churchman, Sally Boxall, Dennis G. McGonagle, and Elena Jones. (2017) Predicting the remaining lifespan and cultivation-related loss of osteogenic capacity of bone marrow multipotential stromal cells applicable across a broad donor age range. Stem Cells International
- Bassil NM, Lloyd JR. (2017) Draft Genome Sequences of Four Alkaliphilic Bacteria Belonging to the Anaerobacillus Genus. Genome Announcemens
- Francesco Muratore, Stefania Croci, Ione Tamagnini, Alessandro Zerbini, Salvatore Bellafiore, Lucia Belloni, Luigi Boiardi, Alessandra Bisagni, Nicolò Pipitone, Maria Parmeggiani, Alberto Cavazza, Carlo Salvarani. (2017) No detection of varicella-zoster virus in temporal arteries of patients with giant cell arteritis. Seminars in Arthritis and Rheumatism
- Denechaud PD, Lopez-Mejia IC, Giralt A, Lai Q, Blanchet E, Delacuisine B, Nicolay BN, Dyson NJ, Bonner C, Pattou F, Annicotte JS, Fajas L. (2016) E2F1 mediates sustained lipogenesis and contributes to hepatic steatosis. The Journal of Clinical Investigation
- Alessandra Iavello, Valeska S.L. Frech, Chiara Gai, Maria Chiara Deregibus, Peter J. Quesenberry And Giovanni Camussi. (2016) Role of Alix in miRNA packaging during extracellular vesicle biogenesis. International Journal of Molecular Medicine
- Stamateris RE, Sharma RB, Kong Y, Ebrahimpour P, Panday D, Ranganath P, Zou B, Levitt H, Parambil NA, O'Donnell CP, Garcia-Ocana A, Alonso LC. (2016) Glucose induces mouse beta cell proliferation via IRS2, mTOR and cyclin D2 but not the insulin receptor. Diabetes
- Boehme SA, Franz-Bacon K, DiTirro DN, Ly TW, Bacon KB. (2016) MAP3K19 Is a Novel Regulator of TGF-β Signaling That Impacts Bleomycin-Induced Lung Injury and Pulmonary Fibrosis. PLoS One
- Liu-Chittenden Y, Patel D, Gaskins K, Giordano TJ, Assie G, Bertherat J, Kebebew E. (2016) Serum RARRES2 Is a Prognostic Marker in Patients with Adrenocortical Carcinoma. The Journal of Clinical Endocrinology & Metabolism.
- Swiderski K, Martins KJ, Chee A, Trieu J, Naim T, Gehrig SM, Baum DM, Brenmoehl J, Chau L, Koopman R, Gregorevic P, Metzger F, Hoeflich A, Lynch GS. (2016) Skeletal muscle-specific overexpression of IGFBP-2 promotes a slower muscle phenotype in healthy but not dystrophic mdx mice and does not affect the dystrophic pathology. Growth Hormone & IGF Research
- Palazzo A, Bluteau O, Messaoudi K, Marangoni F, Chang Y, Souquere S, Pierron G, Lapierre V, Zheng Y, Vainchenker W, Raslova H, Debili N. (2016) The cell division control protein 42 /Src family kinase/Neural Wiskott-Aldrich syndrome protein pathway regulates human proplatelet formation. Journal of Thrombosis and Haemostasis
- Heintz-Buschart A, May P, Laczny CC, Lebrun LA, Bellora C, Krishna A, Wampach L, Schneider JG, Hogan A, de Beaufort C, Wilmes P. (2016) Integrated multi-omics of the human gut microbiome in a case study of familial type 1 diabetes. Nature Microbiology
- Sadakierska-Chudy A, Kotarska A,, Frankowska M, Jastrzębska J, Wydra K, Miszkiel J, Przegaliński E, Filip M. (2016) The Alterations in Mitochondrial DNA Copy Number and Nuclear-Encoded Mitochondrial Genes in Rat Brain Structures after Cocaine Self-Administration. Molecular Neurobiology
- Gregory P Way, Robert J Allaway, Stephanie J Bouley, Camilo E Fadul, Yolanda Sanchez, Casey S Greene. (2016) A machine learning classifier trained on cancer transcriptomes detects NF1 inactivation signal in glioblastoma. bioRxiv
- Boehme SA, Franz-Bacon K, Ludka J, DiTirro DN, Ly TW, Bacon KB. (2016) MAP3K19 Is Overexpressed in COPD and Is a Central Mediator of Cigarette Smoke-Induced Pulmonary Inflammation and Lower Airway Destruction. PLOS ONE
- Enesa K, Moll HP, Luong L, Ferran C, Evans PC. (2015) A20 suppresses vascular inflammation by recruiting proinflammatory signaling molecules to intracellular aggresomes. The FASEB Journal
- Michaela-Kristina Keck. (2015) Functional validation of genetic changes discovered in high-throughput mutational and copy number screens with respect to carcinogenesis and treatment sensitivity for Head and Neck Cancer. Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften
- Damm F, Mylonas E, Cosson A, Yoshida K, Della Valle V, Mouly E, Diop M, Scourzic L, Shiraishi Y, Chiba K, Tanaka H, Miyano S, Kikushige Y, Davi F, Lambert J, Gautheret D, Merle-Beral H, Sutton L, Dessen P, Solary E, Akashi K, Vainchenker W, Mercher T, Dro. (2014) Acquired initiating mutations in early hematopoietic cells of CLL patients. Cancer Discovery
- Michaela K Keck, Zhixiang Zuo, Arun Khattri, Thomas P Stricker, Christopher Brown, Matin Imanguli, Damian Rieke, Katharina Endhardt, Petra Fang, Johannes Bragelmann, Rebecca DeBoer, Mohamed El Dinali, Serdal Aktolga, Zhengdeng Lei, Patrick Tan, Steven G Rozen, Ravi Salgia, Ralph R. Weichselbaum, Mark W. Lingen, Michael D Story, Kie Kian Ang, Ezra EW Cohen, Kevin P. White, Everett E. Vokes, and Tanguy Y. Seiwert. (2014) Integrative analysis of Head and Neck Cancer identifies two biologically distinct HPV and three non-HPV subtypes. American Association for Cancer Research
- El-Mogy M and Haj-Ahmad Y. (2012) Effect of DNA Contaminants on Calcium Phosphate-Based DNA Delivery and Gene Expression. Journal of Biotech Research
- Churchman SM, Ponchel F, Boxall SA, Cuthbert R, Kouroupis D, Roshdy T, Giannoudis PV, Emery P, McGonagle D, Jones EA. (2012) Native CD271(+) multipotential stromal cells (MSCs) have a transcript profile indicative of multiple fates with prominent osteogenic and Wnt pathway signalling activity. Arthritis and Rheumatism
- Manukyana G, Petrekb M, Kriegovab E, Ghazaryana K, Fillerovab R, Boyajyanc A. (2012) Activated phenotype of circulating neutrophils in familial Mediterranean fever. Immunobiology
- Burgdorf SW, Clark CL, Burgdorf JR, Spector DH. (2011) Mutation of Glutamine to Arginine at position 548 of IE2 86 in Human Cytomegalovirus leads to decreased expression of IE2 40, IE2 60, UL83 and UL84 and increased transcription of US8-9 and US29-32. Journal of Virology
- Lawrie A, Hameed A, Chamberlain J, Arnold N, Kennerley A, Hopkinson K, Pickworth J, Kiely DG, Crossman DC, Francis SE. (2011) Paigen Diet-Fed Apolipoprotein E Knockout Mice Develop Severe Pulmonary Hypertension in an Interleukin-1-Dependent Manner. The American Journal of Pathology
- Efimenko A, Starostina E, Kalinina N, Stolzing A. (2011) Angiogenic properties of aged adipose derived mesenchymal stem cells after hypoxic conditioning. Translational Medicine
- Anand Laxmi N., Gunjan G.1 and Prateesh M. (2011) Nucleofection an Efficient Non-viral Transfection Technique for IFN-t-EGFP Gene Expression Study in Sahiwal Cattle Fibroblast Cells. Academic Journals
- Limberg SH, Netter MF, Rolfes C, Rinné S, Schlichthörl G, Zuzarte M, Vassiliou T, Moosdorf R, Wulf H, Daut J, Sachse FB, Decher N. (2011) TASK-1 channels may modulate action potential duration of human atrial cardiomyocytes. Cellular Physiology and Biochemistry
- Simone A. de Bessa Garcia, Michelly C. Pereira and Maria A. Nagai. (2010) Expression Pattern of the Pro-apoptotic Gene PAR-4 During the Morphogenesis of MCF-10A Human Mammary Epithelial Cells. Cancer Microenvironment
- Jones E PhD, English A FIBMS, Churchman SM PhD, Kouroupis D, Boxall SA PhD, Kinsey S, MD, FRCR, Giannoudis PG BSc MB MD FRCS, Emery P MD, FRCP, McGonagle D PhD FRCPI. (2010) Large-scale extraction and characterisation of CD271+ multipotential stromal cells (MSCs) from trabecular bone in health and osteoarthritis: Implications for bone regeneration strategies based on minimally-cultured MSCs. Arthritis and Rhematism
- Rebecca L. Sanders, and Deborah H. Spector. (2010) The Human Cytomegalovirus IE2 86 and IE2 40 Proteins Differentially Regulate UL84 Protein Expression Post-Transcriptionally in the Absence of Other Viral Gene Products. Journal of Virology
- Droin N, Jacquel A, Hendra JB, Racoeur C, Truntzer C, Pecqueur D, Benikhlef N, Ciudad M, Guery L, Jooste V, Dufour E, Fenaux P, Quesnel B, Kosmider O, Fontenay M, Ducoroy P, Solary E. (2010) Alpha-defensins secreted by dysplastic granulocytes inhibit the differentiation of monocytes in chronic myelomonocytic leukemia. Blood
- López MM, Llop P, Olmos A, Marco-Noales E, Cambra M, Bertolini E. (2009) Are molecular tools solving the challenges posed by detection of plant pathogenic bacteria and viruses?. Molecular Biology