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RNA Methyl Sequencing

EpiNext™ 5-mC RNA Bisulfite-Seq Easy Kit

For RNA bisulfite conversion followed by NGS library preparation.

The EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina) is a complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.

  • High sensitivity and efficiency
  • Easy and streamlined procedure
  • Complete conversion
  • Extremely convenient
  • Minimal bias
  • Broad sample suitability

Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for 5-methylcytosine (5-mC) based RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously, followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using low-nanogram quantities of bisulfite converted RNA.

Starting materials can be total RNA isolated from various tissue/cell samples such as fresh and frozen tissues, cultured cells from a flask or microplate, microdissection samples, and body fluid samples, etc. The amount of RNA for each bisulfite reaction can be 5 ng to 500 ng.

Workflow EpiNext™ 5-mC RNA Bisulfite-Seq Easy Kit.

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