RNA Methyl Sequencing
EpiNext™ 5-mC RNA Bisulfite-Seq Easy Kit
For RNA bisulfite conversion followed by NGS library preparation.
The EpiNext 5-mC RNA Bisulfite-Seq Easy Kit (Illumina) is a complete set of optimized reagents designed for easily carrying out RNA bisulfite conversion, followed by a "post-bisulfite" library preparation process for Illumina platform-based bisulfite sequencing, all in one kit.
- High sensitivity and efficiency
- Easy and streamlined procedure
- Complete conversion
- Extremely convenient
- Minimal bias
- Broad sample suitability
Intended applications include whole transcriptome RNA bisulfite sequencing and various other RNA bisulfite-based next generation sequencing techniques for 5-methylcytosine (5-mC) based RNA methylation analysis. The optimized protocol and components of the kit allow the RNA to be bisulfite converted and fragmented simultaneously, followed by quick non-barcoded (singleplexed) and barcoded (multiplexed) library construction using low-nanogram quantities of bisulfite converted RNA.
Starting materials can be total RNA isolated from various tissue/cell samples such as fresh and frozen tissues, cultured cells from a flask or microplate, microdissection samples, and body fluid samples, etc. The amount of RNA for each bisulfite reaction can be 5 ng to 500 ng.
Workflow EpiNext™ 5-mC RNA Bisulfite-Seq Easy Kit.