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mRNA Sequencing

RapidSeq Directional mRNA Sample Prep Kit

Generate strand specific mRNA libraries compatible with Illumina.

The RapidSeq mRNA Sample Prep Kit was developed to prepare directional NGS libraries for subsequent cluster generation using purified mRNA as start material. The protocol includes steps for mRNA fragmentation, purification, adapter ligation, reverse transcription, PCR amplification, and DNA fragment enrichment to generate strand specific library product compatible with Illumina NGS platform

Strand-specific RNA-seq improves on standard RNA-seq in three ways: accurately identifying antisense transcripts, determining the transcribed strand of non-coding RNAs and demarcating the boundaries of closely situated or overlapping genes.

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DNA1000 chip trace of the final library from a human lung tissue mRNA sample.

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