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KO Cell Lines & Lysates


Gene Knockout Cell Lines

A gene knockout (KO) cell line is a powerful research tool that allows the identification of a gene’s function through the loss-of-function phenotype. Our partner EdiGene has developed an innovative and highly efficient CRISPR/Cas9 approach to design, develop and validate gene knockout in most commonly used human cells such as HEK293T, HeLa, HCT116, HepG2, A549 and Jurkat to speed up your research and save research money.
Over 2,000 ready-made knockout cell lines (Edi-G KO Cell Lines) are provided. The included parental control enables disease-related gene function and pathway analysis in isogenic cell lines. To validate gene knockout, the target region of each Edi-G KO Cell Line is sequenced twice to ensure each allele of the target gene has an insertion/deletion mutation but not an in-frame mutation.

  • Over 2,000 validated clonal knockout cell lines
  • Gene knockout in commonly used cell lines like HEK293T or HeLa
  • 100% knockout and complete loss of gene function
  • Sequencing data provided in the data sheet
  • Parental control included for loss-of-function comparison
  • Corresponding cell lysates available

Knockout cell lines for hot genes such as BRAF, EGFR, KRAS, NRAS, NOTCH1-3, PD-1, PD-L1 PIK3, TP53, etc. are offered. Find your Edi-G knockout cell line(s) by using the BioCat KO Cell Line search tool above, or browse the Edi-G knockout cell lines grouped by pathway / gene families, see right column. Not finding the KO cell line of your interest? Any questions about our KO cell lines? Please contact us, we are happy to help you.


Applications

Edi-G KO Cell Lines and related products are ideally suited for:

  • Gene function and biological pathway analysis
  • Biomarker discovery and validation
  • Drug target identification and validation
  • Cancer development and progression studies
  • Polyclonal and monoclonal antibody validation

Gene Knockout Cell Lysates

Validating an antibody’s specificity is crucial to ensure the absence of non-specific binding and, therefore, the highest level of functionality. Testing antibody performance using genetically modified samples is one way to verify that an antibody recognizes a specific target. EdiGene’s KO Cell Lysates are designed to be used as negative controls for antibody testing by Western Blot analysis and ultimately to validate antibody specificity.


MET Monoclonal Antibody Validation using MET Knockout Cell Lysate

MET Antibody Validation

10 ug each of the Edi-G MET Knockout Cell Lysate (Cat# # L0018998301-EG) and of the parental HeLa Cell Lysate were used in Western Blot analysis to show the specificity of a MET Monoclonal Antibody (Cat# UM800082-OR). MET was detected at ~145 kDa. The results confirm the specificity of the antibody. Data were normalized using an anti-ß-actin antibody as loading control (1:500).

The knockout cell derivatives like Edi-G KO Cell Lysates provide further options and flexibility for the genome editing workflow. Representing a powerful tool for the identification, validation and confirmation of gene functions in cancer biology and signaling pathways, Edi-G KO Cell Lines and Lysates are rapidly becoming industry leading standards for antibody validation and quality control.


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