siRNA Transfection in Mammalian CellsGeneSilencer siRNA Transfection Reagent
• Efficient transfection of siRNA into mammalian cells.
• High siRNA transfection efficiency
• Functional gene silencing post siRNA delivery
• Compatibility with diverse growth conditions (with and without serum)
• Low cytotoxicity
• Easy-to-use protocols for both adherent and suspension cells
Efficient siRNA delivery is critical for successful gene silencing. GeneSilencer siRNA Transfection Reagent is a novel cationic lipid formulation specifically designed for efficient delivery of siRNAs (small interfering RNAs) into a wide variety of cell types including primary cells, see table below.
GeneSilencer siRNA Transfection Reagent has a low toxicity profile while maintaining high transfection efficiencies - in some cases up to 90%. This contrasts sharply with nuclear electroporation methods, which can result in very low viabilities, and viral methods, which are cumbersome, time-consuming, and raise potential safety issues.
Easy-to-use protocols for siRNA delivery into both adherent and suspension cells are available. GeneSilencer siRNA Transfection Reagent is compatible with diverse growth conditions and works well in the presence or absence of serum.
Transfection of GFP-specific siRNA using GeneSilencer siRNA Transfection Reagent.
293 cells expressing GFP after transient transfection with gWiz GFP plasmid (A). When cells were co-transfected with gWiz GFP plasmid and anti-GFP siRNA using GeneSilencer siRNA Transfection Reagent, GFP expression was effectively suppressed (B). Cells were imaged 48 hours post-transfection.
For references featuring the use of GeneSilencer® siRNA Transfection Reagent, see Citation Search
|GeneSilencer siRNA Transfection Reagent (0.2 ml)||T500020-GL||107 rxns (100 ng siRNA/transfection)||217 €||DETAILS||Add to Cart|
|GeneSilencer siRNA Transfection Reagent (0.75 ml)||T500750-GL||400 rxns (100 ng siRNA/transfection)||483 €||DETAILS||Add to Cart|
|GeneSilencer siRNA Transfection Reagent (5 x 0.75 ml)||T505750-GL||2000 rxns (100 ng siRNA/transfection)||1831 €||DETAILS||Add to Cart|
|RNAifectin siRNA Transfection Reagent (1 ml) (Multiple Cationic Polymers)||G073-ABM||250 rxns (1 ug siRNA/transfection)||235 €||DETAILS||Add to Cart|
|siTran 2.0 Transfection Reagent designed for siRNA Duplex||TT320001-OR||0,5 ml||220 €||DETAILS||Add to Cart|