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Retroviral Expression Systems

• Achieve average titer of 10^7 infectious units/mL with transient transfection

• Produce ecotropic, amphotropic or pantropic retrovirus

• 3 formats: Optimized for ES & EC cells, for Hematopoietic Stem Cells, or Universal Gene Expression System


Retroviral vectors are useful for delivering genes of interest into dividing target cells where integration into the genome is desired. However, traditional retroviral expression technologies result in low viral titers, making gene expression studies challenging.
Platinum Retroviral Expression Systems from Cellbiolabs incorporate superior packaging cell lines and vector technologies to produce high-titer virus with a single plasmid transfection.
Each system includes one of Cellbiolabs´exclusive Platinum packaging cell lines: Plat-E (ecotropic, Plat-A (amphotropic), or Plat-GP (pantropic). All 3 cell lines contain the gag and pol genes; Plat-E and Plat-A cells also contain the envelope protein.
NOTE: Platinum Retroviral Packaging Cells are available for sale to academic, government and non-profit research laboratories. All other purchasers require a commercial license for all fields including research use. Please contact info@biocat.com to ask for licensing conditions.

Clone your gene of interest into the vector provided and transfect into the packaging cells (for pantropic virus, cotransfect the included VSV-G plasmid).



 

 

Description Cat# Size Price    
Platinum Retroviral Gene Expression System for ES (Embryonal Stem) and EC (Embryonal Carcinoma) Cells, Amphotrophic: Kit incl. Platinum-A Packaging Cell Line, Expression and Control Vectors VPK-304-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Gene Expression System for ES (Embryonal Stem) and EC (Embryonal Carcinoma) Cells, Ecotrophic: Kit incl. Platinum-E Packaging Cell Line, Expression and Control Vectors VPK-303-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Gene Expression System for ES (Embryonal Stem) and EC (Embryonal Carcinoma) Cells, Pantrophic: Kit incl. Platinum-GP Packaging Cell Line, Expression and Control Vectors VPK-305-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Gene Expression System for HSC / Hematopoietic Stem Cells, Amphotrophic: Kit incl. Platinum-A Packaging Cell Line, Expression and Control Vectors VPK-307-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Gene Expression System for HSC / Hematopoietic Stem Cells, Ecotrophic: Kit incl. Platinum-E Packaging Cell Line, Expression and Control Vectors VPK-306-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Gene Expression System for HSC / Hematopoietic Stem Cells, Pantrophic: Kit incl. Platinum-GP Packaging Cell Line, Expression and Control Vectors VPK-308-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Universal Gene Expression System, Amphotrophic: Kit incl. Platinum-A Packaging Cell Line, Expression and Control Vectors VPK-301-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Universal Gene Expression System, Ecotrophic: Kit incl. Platinum-E Packaging Cell Line, Expression and Control Vectors VPK-300-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 
Platinum Retroviral Universal Gene Expression System, Pantrophic: Kit incl. Platinum-GP Packaging Cell Line, Expression and Control Vectors VPK-302-GVO-CB 3 x 10^6 cells and 10 ug of each vector 1112 € DETAILS   Add to Cart 

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