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Home -> Genomics -> Reporter Assays -> Dual Reporter Assays: Gaussia Luciferase and SEAP (Secreted Reporters)

Dual Reporter Assays: Gaussia Luciferase and SEAP (Secreted Reporters)

The Secrete-Pair Dual Luminescence Assay Kit developed by GeneCopoeia is designed to analyze the activities of Gaussia Luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) in a dual-reporter system. Both GLuc and SEAP are secreted reporter proteins, permitting detection without cell lysis. Secrete-Pair measures dual reporter signals and allows transfection normalization.
A single GLuc reporter system is also available.

Live cell assays - Secreted GLuc and SEAP make cell lysis unnecessary. The same sample can be assayed multiple times for different time points, environmental conditions, etc., reducing sample-to-sample variation. Ideal for high-throughput applications.
Dual-reporter detection - SEAP allows normalization of GLuc signal for greater accuracy.
Sensitive and robust system - GLuc is 1,000 times more sensitive than firefly and Renilla luciferases.
Flexible assay conditions - Two robust buffer conditions are provided for GLuc assays: 1) Buffer for greater stability retains more than 90% of signal within the first 10 minutes, extending the half-life of luminescence to 30 minutes; 2) Buffer for higher sensitivity for detecting low GLuc expression.
Clone and vector compatibility - Compatible with GeneCopoeia´s GLuc-ON promoter reporter clones, miTarget miRNA target clones, GLuc-ON Transcriptional Response Element (TRE) clones, and cloning vectors.

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Product Details

 

 

Description Cat# Size Price    
Secrete-Pair Dual Luminescence Assay Kit - detects Gaussia luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) LF031-GC 100 reactions 163 € DETAILS   Add to Cart 
Secrete-Pair Dual Luminescence Assay Kit - detects Gaussia luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) LF032-GC 300 reactions 409 € DETAILS   Add to Cart 
Secrete-Pair Dual Luminescence Assay Kit - detects Gaussia luciferase (GLuc) and Secreted Alkaline Phosphatase (SEAP) LF033-GC 1000 reactions 1004 € DETAILS   Add to Cart 

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