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TaqMan Probe Synthesis and Purification Service

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Over the last decade, TaqMan probes have become the traditional linear, dual-labeled FRET probes of choice now incorporating a Black Hole Quencher dye and a fluorophore reporter molecule covalently linked to either the 3´ or 5´ end of a 20 to 30 bp oligo. TaqMan probes are routinely used to detect the presence and quantify the amount of specific target sequences by employing the 5´-> 3´ exonuclease activity of Taq polymerase and the subsequent increase in fluorescence detected during repeated amplification cycles. The TaqMan assay offers a sensitive method to determine the presence or absence of specific target sequences.

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TaqMan Probe
Dual-labeled, linear, sequence-specific probe, used with a pair of forward and reverse primers.

The 5´ nuclease assay has now become a staple of real-time PCR quantification techniques. In the 5´ nuclease assay, fluorescent signals are generated from the exponential range of the reaction, where component concentrations are not limiting. As a result, initial template levels can be determined with high accuracy.

Multiplex assays are now possible that can detect several targets using multiple spectrally resolved fluorescent probes, see Multiplexing Recommendations Table (link below).

Custom TaqMan probes labeled with a variety of fluorophores and quenchers to accommodate all assays and thermocyclers are offered, see table below.
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Real-Time PCR Dyes from Biosearch Technologies


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Multiplexing Recommendations Table

 

 

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Description Cat# Size Price    
Custom Synthesis and Cartridge Purification of Primer (up to 30 bases) with TaqMan Probe Purchase PPC-1-BS 1 umol Scale (provides 225-450 nmol pure primer) 83 € DETAILS   Add to Cart 
Custom Synthesis and Cartridge Purification of Primer (up to 30 bases) with TaqMan Probe Purchase PPC-2-BS 200 nmol Scale (provides 45-90 nmol pure primer) 75 € DETAILS   Add to Cart 
Custom Synthesis and Cartridge Purification of Primer (up to 30 bases) with TaqMan Probe Purchase PPC-5-BS 50 nmol Scale (provides 11,25-22,5 nmol pure primer) 73 € DETAILS   Add to Cart 
Custom Synthesis and Desalting of Primer (up to 30 bases) with TaqMan Probe Purchase PP-1-BS 1 umol Scale (provides 400-800 nmol pure primer) 78 € DETAILS   Add to Cart 
Custom Synthesis and Desalting of Primer (up to 30 bases) with TaqMan Probe Purchase PP-2-BS 200 nmol Scale (provides 80-160 nmol pure primer) 70 € DETAILS   Add to Cart 
Custom Synthesis and Desalting of Primer (up to 30 bases) with TaqMan Probe Purchase PP-5-BS 50 nmol Scale (provides 20-40 nmol pure primer) 65 € DETAILS   Add to Cart 
Dual Labeled Probe: 3' Pulsar 650 Reporter (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) DLO-B2P6-1-BS 1 umol Scale (provides 40 nmol pure probe) 779 € DETAILS   Add to Cart 
Dual Labeled Probe: 3' Pulsar 650 Reporter (Emission 650 nm) 5' BHQ-2 (Black Hole Quencher) DLO-B2P6-2-BS 200 nmol Scale (provides 10 nmol pure probe) 565 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) DLO-CGB1-1-BS 1 umol Scale (provides > 60 nmol pure probe) 501 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) DLO-CGB1-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 378 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Gold 540 Fluorophore Reporter (Emission 544 nm) 3' BHQ-1 (Black Hole Quencher) DLO-CGB1-5-BS 50 nmol Scale (provides > 10 nmol pure probe) 312 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) DLO-COB1-1-BS 1 umol Scale (provides > 60 nmol pure probe) 501 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) DLO-COB1-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 378 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Orange 560 Reporter (Emission 559 nm) 3' BHQ-1 (Black Hole Quencher) DLO-COB1-5-BS 50 nmol Scale (provides > 10 nmol pure probe) 312 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 590 Reporter (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) DLO-C590B2-1-BS 1 umol Scale (provides > 60 nmol pure probe) 490 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 590 Reporter (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) DLO-C590B2-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 373 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 590 Reporter (Emission 591 nm) 3' BHQ-2 (Black Hole Quencher) DLO-C590B2-5-BS 50 nmol Scale (provides > 10 nmol pure probe) 312 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 610 Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) DLO-CAB2-1-BS 1 umol Scale (provides > 60 nmol pure probe) 582 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 610 Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) DLO-CAB2-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 420 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 610 Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) DLO-CAB2-5-BS 50 nmol Scale (provides > 10 nmol pure probe) 334 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 635 Reporter (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) DLO-C635B2-1-BS 1 umol Scale (provides > 40 nmol pure probe) 662 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 635 Reporter (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) DLO-C635B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 485 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' CAL Fluor Red 635 Reporter (Emission 637 nm) 3' BHQ-2 (Black Hole Quencher) DLO-C635B2-5-BS 50 nmol Scale (provides > 3 nmol pure probe) 377 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) / Internal T-BHQ-1 (Black Hole Quencher) / 3' C3 DLO-F(T-B1)-1-BS 1 umol Scale (provides > 30 nmol pure probe) 624 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) / Internal T-BHQ-1 (Black Hole Quencher) / 3' C3 DLO-F(T-B1)-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 398 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) DLO-FB1-1-BS 1 umol Scale (provides > 60 nmol pure probe) 463 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) DLO-FB1-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 350 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' BHQ-1 (Black Hole Quencher) DLO-FB1-5-BS 100 nmol Scale (provides > 10 nmol pure probe) 275 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' TAMRA (Quencher Emission 583 nm) DLO-FT-1-BS 1 umol Scale (provides > 60 nmol pure probe) 463 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' TAMRA (Quencher Emission 583 nm) DLO-FT-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 350 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' FAM Fluorophore Reporter (Emission 520 nm) 3' TAMRA (Quencher Emission 583 nm) DLO-FT-5-BS 100 nmol Scale (provides > 10 nmol pure probe) 275 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) DLO-HB1-1-BS 1 umol Scale (provides > 60 nmol pure probe) 554 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) DLO-HB1-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 415 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' HEX Fluorophore Reporter (Emission 556 nm) 3' BHQ-1 (Black Hole Quencher) DLO-HB1-5-BS 50 nmol Scale (provides > 10 nmol pure probe) 323 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' JOE Fluorophore Reporter (Emission 555 nm) 3' BHQ-1 (Black Hole Quencher) DLO-JB1-1-BS 1 umol Scale (provides > 30 nmol pure probe) 758 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' JOE Fluorophore Reporter (Emission 555 nm) 3' BHQ-1 (Black Hole Quencher) DLO-JB1-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 596 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 570 Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q5B2-1-BS 1 umol Scale (provides > 60 nmol pure probe) 554 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 570 Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q5B2-2-BS 200 nmol Scale (provides > 25 nmol pure probe) 420 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 570 Reporter (Emission 566 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q5B2-5-BS 50 nmol Scale (provides > 10 nmol pure probe) 334 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 670 Reporter (Emission 667 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q6B2-1-BS 1 umol Scale (provides > 40 nmol pure probe) 582 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 670 Reporter (Emission 667 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q6B2-2-BS 200 nmol Scale (provides > 20 nmol pure probe) 420 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 670 Reporter (Emission 667 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q6B2-5-BS 50 nmol Scale (provides 10 nmol pure probe) 349 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 705 Reporter (Emission 705 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q7B2-1-BS 1 umol Scale (provides > 40 nmol pure probe) 582 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 705 Reporter (Emission 705 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q7B2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 420 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 705 Reporter (Emission 705 nm) 3' BHQ-2 (Black Hole Quencher) DLO-Q7B2-5-BS 50 nmol Scale (provides >3 nmol pure probe) 349 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 705 Reporter (Emission 705 nm) 3' BHQ-3 (Black Hole Quencher) DLO-Q7B3-1-BS 1 umol Scale (provides > 40 nmol pure probe) 582 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 705 Reporter (Emission 705 nm) 3' BHQ-3 (Black Hole Quencher) DLO-Q7B3-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 420 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' Quasar 705 Reporter (Emission 705 nm) 3' BHQ-3 (Black Hole Quencher) DLO-Q7B3-5-BS 50 nmol Scale (provides >3 nmol pure probe) 349 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' ROX Fluorophore Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) DLO-RB2-1-BS 1 umol Scale (provides > 30 nmol pure probe) 758 € DETAILS   Add to Cart 
Dual Labeled Probe: 5' ROX Fluorophore Reporter (Emission 610 nm) 3' BHQ-2 (Black Hole Quencher) DLO-RB2-2-BS 200 nmol Scale (provides > 10 nmol pure probe) 596 € DETAILS   Add to Cart 
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