Black Hole Scorpions Synthesis and Purification Service

Unlike TaqMan probes and Molecular Beacons which are based on a bi-molecular probing mechanism, Biosearch´s Black Hole Scorpions utilize a uni-molecular mechanism that does not require enzymatic cleavage of the probe during PCR cycling. This provides a number of important advantages over other PCR probe formats:

• Measurably faster reactions
• Enhanced sensitivity
• Simplified assay design
• Exceptional specificity
• Excellent S:N
• Easily multiplexed

These qualities make Black Hole Scorpions valuable tools for:
- Rapid, Real-time qPCR
- Endpoint PCR
- SNP Detection
- Gene Quantification
- Environmental Analysis


Black Hole Scorpion Primer
Dual-labeled hairpin probe with sequence-specific loop and primer, used with one reverse primer.

Black Hole Scorpions Architecture
Black Hole Scorpions are probe/primer hybrids whose design, unlike other FRET probes, is such that they emit light only when bound to their complementary target sequence during PCR amplification. Black Hole Scorpions incorporate two distinct structures: 1) a target-specific DNA probing sequence and 2) a target-specific PCR primer.

DNA Probing Sequence
The DNA probing sequence incorporates a self-complementary stem sequence at the 3´ and 5´ ends that holds the amplicon specific region in a hairpin loop. A fluorophore molecule is covalently linked to the 5´ end and, the dark quencher, a Black Hole Quencher dye, is linked to the 3´ end of the loop structure. The fluorophore and BHQ dye are held in close physical proximity by the stem structure thereby ensuring efficient suppression of fluorescence prior to thermocycling.

Target-specific Primer
The target-specific PCR primer is covalently linked to the 3´ end of the DNA probing sequence through a blocking molecule. This molecule prevents polymerase read-through thereby eliminating the formation of primer dimers or mis-priming.

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Real-Time PCR Dyes from Biosearch Technologies

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Multiplexing Recommendations Table

 

 

Description	Cat#	Size	Price
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Orange 560 (Emission 559 nm) Internal BHQ-1/HEG (Black Hole Quencher)	SCP-COB1-1-BS	1 umol Scale (provides > 30 nmol pure probe)	801 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Orange 560 (Emission 559 nm) Internal BHQ-1/HEG (Black Hole Quencher)	SCP-COB1-2-BS	200 nmol Scale (provides > 10 nmol pure probe)	603 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Red 590 (Emission 591 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-C590B2-1-BS	1 umol Scale (provides > 30 nmol pure probe)	801 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Red 590 (Emission 591 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-C590B2-2-BS	200 nmol Scale (provides > 10 nmol pure probe)	603 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Red 610 (Emission 610 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-CAB2-1-BS	1 umol Scale (provides > 30 nmol pure probe)	801 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Red 610 (Emission 610 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-CAB2-2-BS	200 nmol Scale (provides > 10 nmol pure probe)	603 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Red 635 (Emission 637 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-C635B2-1-BS	1 umol Scale (provides > 15 nmol pure probe)	801 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' CAL Fluor Red 635 (Emission 637 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-C635B2-2-BS	200 nmol Scale (provides > 5 nmol pure probe)	603 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' FAM (Emission 520 nm) Internal BHQ-1/HEG (Black Hole Quencher)	SCP-FB1-1-BS	1 umol Scale (provides > 30 nmol pure probe)	749 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' FAM (Emission 520 nm) Internal BHQ-1/HEG (Black Hole Quencher)	SCP-FB1-2-BS	200 nmol Scale (provides > 10 nmol pure probe)	551 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' Qasar 570 (Emission 566 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-Q5B2-1-BS	1 umol Scale (provides > 30 nmol pure probe)	801 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' Qasar 570 (Emission 566 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-Q5B2-2-BS	200 nmol Scale (provides > 10 nmol pure probe)	655 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' Qasar 670 (Emission 670 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-Q6B2-1-BS	1 umol Scale (provides > 15 nmol pure probe)	853 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' Qasar 670 (Emission 670 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-Q6B2-2-BS	200 nmol Scale (provides > 5 nmol pure probe)	707 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' TAMRA (Emission 583 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-TB2-1-BS	1 umol Scale (provides > 30 nmol pure probe)	801 €	DETAILS	Add to Cart
Black Hole Scorpions Unimolecular Probe: 5' TAMRA (Emission 583 nm) Internal BHQ-2/HEG (Black Hole Quencher)	SCP-TB2-2-BS	200 nmol Scale (provides > 10 nmol pure probe)	655 €	DETAILS	Add to Cart
Custom Synthesis and Purification of Reverse Primer (up to 30 bases) ordered together with Black Hole Scorpions Probe	SCP-P1-1-BS	1 umol scale (provides > 30 nmol pure primer)	114 €	DETAILS	Add to Cart
Custom Synthesis and Purification of Reverse Primer (up to 30 bases) ordered together with Black Hole Scorpions Probe	SCP-P1-2-BS	200 nmol scale (provides > 10 nmol pure primer)	112 €	DETAILS	Add to Cart