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Probe-based qPCR Kits

TaqProbe qPCR Mastermix formulations of our partner ABM are optimized for different real-time PCR instruments. Please use the following table as a guideline for the selection of qPCR Mastermix appropriate for your particular instrument model.

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GeneCopoeias´ BlazeTaq™ Probe qPCR mix is designed for highly sensitive and accurate quantitative PCR (qPCR) that uses hydrolysis probes and DNA as starting material. The kit is supplied with a ready-to-use 5X master mix with a hot-start Taq DNA polymerase, dNTP and all optimized buffer components. Tests show that the BlazeTaq™ Probe qPCR Mix is compatible with diverse real-time PCR instruments and outperforms competitors´ products on the sensitivity and specificity of cDNA and genomic DNA detection.

BlazeTaq™ Probe qPCR Mix allows sensitive and potent target DNA detection and quantification using hydrolysis probes. cDNA or genomic DNA as template is amplified by a hot-start Taq DNA polymerase. During DNA amplification, the target-specific probes bound to the target site on DNA are cleaved by the 5´-3´ exonuclease of Taq DNA polymerase. Upon cleavage, the unquenched probes generate fluorescence for real-time quantification.


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qPCR targeting ACTB (A), and B2M (B) using the BlazeTaq Probe qPCR (red) kit and competitor´s Taqman qPCR kit (blue) with cDNA from HeLa RNA extract ranging from 100 ng to 1 pg in duplicates.


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Performance comparison of BlazeTaq Probe qPCR kit with competitor´s qPCR kit by the amplification of B2M from cDNA of HeLa cells.



Please see individual products for more information.

Related Links

Probe-based qRT-PCR Kits
SYBR Green qPCR Kits
SYBR Green qRT-PCR Kits
EvaGreen qPCR Kits
EvaGreen qRT-PCR Kits

 

 

Description Cat# Size Price    
2 x qPCR Mastermix for Probe based qPCR with Regular ROX (4 x 1.25 ml) MasterMix-P-ABM 500 rxns (in 20 ul volumes) 270 € DETAILS   Add to Cart 
5 x qPCR Mastermix for Probe based qPCR with Regular ROX (4 x 1 ml) MasterMix-5P-ABM 1000 rxns (in 20 ul volumes) 382 € DETAILS   Add to Cart 
2 x qPCR Mastermix for Probe based qPCR with Low ROX (4 x 1.25 ml) MasterMix-PL-ABM 500 rxns (in 20 ul volumes) 270 € DETAILS   Add to Cart 
5 x qPCR Mastermix for Probe based qPCR with Low ROX (4 x 1 ml) MasterMix-5PL-ABM 1000 rxns (in 20 ul volumes) 382 € DETAILS   Add to Cart 
2 x qPCR Mastermix for Probe based qPCR for iCycler (4 x 1.25 ml) MasterMix-PC-ABM 500 rxns (in 20 ul volumes) 270 € DETAILS   Add to Cart 
5 x qPCR Mastermix for Probe based qPCR for iCycler (4 x 1 ml) Mastermix-5PC-ABM 1000 rxns (in 20 ul volumes) 382 € DETAILS   Add to Cart 
2 x qPCR Mastermix for Probe based qPCR without ROX (4 x 1.25 ml) MasterMix-PS-ABM 500 rxns (in 20 ul volumes) 270 € DETAILS   Add to Cart 
5 x qPCR Mastermix for Probe based qPCR without ROX (4 x 1 ml) MasterMix-5PS-ABM 1000 rxns (in 20 ul volumes) 382 € DETAILS   Add to Cart 
2 x qPCR Mastermix for Probe based qPCR, Multiplex Applications (4 x 1.25 ml) MasterMix-PM-ABM 500 rxns (in 20 ul volumes) 270 € DETAILS   Add to Cart 
5 x qPCR Mastermix for Probe based qPCR, Multiplex Applications (4 x 1 ml) MasterMix-5PM-ABM 1000 rxns (in 20 ul volumes) 382 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (with ROX) QP035-GC 100 qPCR reactions 120 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (with ROX) QP036-GC 200 qPCR reactions 213 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (with ROX) QP037-GC 600 qPCR reactions 398 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (with ROX) QP039-GC 1000 qPCR reactions 810 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (without ROX) QP045-GC 100 qPCR reactions 120 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (without ROX) QP046-GC 200 qPCR reactions 213 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (without ROX) QP047-GC 600 qPCR reactions 398 € DETAILS   Add to Cart 
BlazeTaq Probe qPCR Mix (without ROX) QP049-GC 1000 qPCR reactions 810 € DETAILS   Add to Cart 
Pro qPCR SuperMix Kit K5053200-BC 200 rxn 240 € DETAILS   Add to Cart 
Pro qPCR SuperMix Kit K5053400-BC 400 rxn 357 € DETAILS   Add to Cart 

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